제품 > 항체 > 다중클론항체(pAb)

SD1 Rabbit pAb (A20664)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - SD1 Rabbit pAb (A20664)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using SD1 Rabbit pAb (A20664) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product nameSD1 Rabbit pAb
Catalog No.A20664
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Key oxidase enzyme in the biosynthesis of gibberellin
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-140 of Oryza sativa SD1. (Q0JH50).
SequenceMVAEHPTPPQPHQPPPMDSTAGSGIAAPAAAAVCDLRMEPKIPEPFVWPNGDARPASAAELDMPVVDVGVLRDGDAEGLRRAAAQVAAACATHGFFQVSEHGVDAALARAALDGASDFFRLPLAEKRRARRVPGTVSGYT
Gene ID
Swiss Prot
Synonymssd1; GA20; Sd-1; 20ox2; C20ox2; SD1-2E; GA20OX2; Os20ox2; osGA20ox2; SD1
Calculated MW43kDa
Observed MW43kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesleaves, stems
Cellular location
    ABclonal:Western blot - SD1 Rabbit pAb (A20664)}

    Western blot - SD1 Rabbit pAb (A20664)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using SD1 Rabbit pAb (A20664) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)