제품 > 항체 > 다중클론항체(pAb)

SDHC Rabbit pAb (A21040)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SDHC Rabbit pAb (A21040)

Western blot analysis of various lysates using SDHC Rabbit pAb (A21040) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - SDHC Rabbit pAb (A21040)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using SDHC Rabbit pAb (A21040) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - SDHC Rabbit pAb (A21040)

Immunofluorescence analysis of A-431 cells using SDHC Rabbit pAb (A21040) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SDHC Rabbit pAb (A21040)

Immunofluorescence analysis of HeLa cells using SDHC Rabbit pAb (A21040) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SDHC Rabbit pAb (A21040)

Immunofluorescence analysis of NIH/3T3 cells using SDHC Rabbit pAb (A21040) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameSDHC Rabbit pAb
Catalog No.A21040
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes one of four nuclear-encoded subunits that comprise succinate dehydrogenase, also known as mitochondrial complex II, a key enzyme complex of the tricarboxylic acid cycle and aerobic respiratory chains of mitochondria. The encoded protein is one of two integral membrane proteins that anchor other subunits of the complex, which form the catalytic core, to the inner mitochondrial membrane. There are several related pseudogenes for this gene on different chromosomes. Mutations in this gene have been associated with paragangliomas. Alternatively spliced transcript variants have been described.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human SDHC (NP_002992.1).
SequenceMAALLLRHVGRHCLRAHFSPQLCIRNAVPLGTTAKEEMERFWNKNIGSNRPLSPHITIYSWSLPMAMSICHRGTGIALSAGVSLFGMSALLLPGNFESYL
Gene ID
Swiss Prot
SynonymsCYBL; PGL3; QPS1; SDH3; CYB560; SDHC
Calculated MW19kDa
Observed MW12kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HepG2, Mouse liver, Rat liver
Cellular locationMitochondrion inner membrane, Multi-pass membrane protein.
Customer validation

ChIP(Homo sapiens)

IF(Homo sapiens,Mus musculus)

IHC(Homo sapiens,Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - SDHC Rabbit pAb (A21040)}

Western blot - SDHC Rabbit pAb (A21040)

Western blot analysis of various lysates using SDHC Rabbit pAb (A21040) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - SDHC Rabbit pAb (A21040)}

Immunohistochemistry - SDHC Rabbit pAb (A21040)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using SDHC Rabbit pAb (A21040) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - SDHC Rabbit pAb (A21040)}

Immunofluorescence - SDHC Rabbit pAb (A21040)

Immunofluorescence analysis of A-431 cells using SDHC Rabbit pAb (A21040) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SDHC Rabbit pAb (A21040)}

Immunofluorescence - SDHC Rabbit pAb (A21040)

Immunofluorescence analysis of HeLa cells using SDHC Rabbit pAb (A21040) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SDHC Rabbit pAb (A21040)}

Immunofluorescence - SDHC Rabbit pAb (A21040)

Immunofluorescence analysis of NIH/3T3 cells using SDHC Rabbit pAb (A21040) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A21040? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)