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SEMA4F Rabbit pAb (A10432)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SEMA4F Rabbit pAb (A10432)

Western blot analysis of various lysates using SEMA4F Rabbit pAb (A10432) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - SEMA4F Rabbit pAb (A10432)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using SEMA4F Rabbit pAb (A10432) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameSEMA4F Rabbit pAb
Catalog No.A10432
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transmembrane class IV semaphorin family protein, which plays a role in neural development. This gene may be involved in neurogenesis in prostate cancer, the development of neurofibromas, and breast cancer tumorigenesis. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 280-420 of human SEMA4F (NP_004254.2).
SequenceRKTLQQRWTTFLKADLLCPGPEHGRASSVLQDVAVLRPELGAGTPIFYGIFSSQWEGATISAVCAFRPQDIRTVLNGPFRELKHDCNRGLPVVDNDVPQPRPGECITNNMKLRHFGSSLSLPDRVLTFIRDHPLMDRPVFP
Gene ID
Swiss Prot
SynonymsS4F; SEMAM; SEMAW; M-SEMA; PRO2353; m-Sema-M; SEMA4F
Calculated MW84kDa
Observed MW83kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesSH-SY5Y, HepG2, A-549, Mouse brain, Mouse liver, Mouse lung, Rat brain, Rat liver
Cellular locationMembrane, Single-pass type I membrane protein

Documents

Certificate of Compliance

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    ABclonal:Western blot - SEMA4F Rabbit pAb (A10432)}

    Western blot - SEMA4F Rabbit pAb (A10432)

    Western blot analysis of various lysates using SEMA4F Rabbit pAb (A10432) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - SEMA4F Rabbit pAb (A10432)}

    Immunohistochemistry - SEMA4F Rabbit pAb (A10432)

    Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using SEMA4F Rabbit pAb (A10432) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.