제품 > 항체 > 다중클론항체(pAb)

SERPINI2 Rabbit pAb (A11732)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - SERPINI2 Rabbit pAb (A11732)

Western blot analysis of various lysates using SERPINI2 Rabbit pAb (A11732) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - SERPINI2 Rabbit pAb (A11732)

Immunofluorescence analysis of paraffin-embedded rat pancreas using SERPINI2 Rabbit pAb (A11732) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SERPINI2 Rabbit pAb (A11732)

Immunofluorescence analysis of paraffin-embedded mouse pancreas using SERPINI2 Rabbit pAb (A11732) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameSERPINI2 Rabbit pAb
Catalog No.A11732
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The gene encodes a member of a family of proteins that acts as inhibitors of serine proteases. These proteins function in the regulation of a variety of physiological processes, including coagulation, fibrinolysis, development, malignancy, and inflammation. Expression of the encoded protein may be downregulated during pancreatic carcinogenesis. Alternative splicing results in multiple transcript variants for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 56-405 of human SERPINI2 (NP_006208.1).
SequenceLEMVQLGAKGKAQQQIRQTLKQQETSAGEEFFVLKSFFSAISEKKQEFTFNLANALYLQEGFTVKEQYLHGNKEFFQSAIKLVDFQDAKACAEMISTWVERKTDGKIKDMFSGEEFGPLTRLVLVNAIYFKGDWKQKFRKEDTQLINFTKKNGSTVKIPMMKALLRTKYGYFSESSLNYQVLELSYKGDEFSLIIILPAEGMDIEEVEKLITAQQILKWLSEMQEEEVEISLPRFKVEQKVDFKDVLYSLNITEIFSGGCDLSGITDSSEVYVSQVTQKVFFEINEDGSEAATSTGIHIPVIMSLAQSQFIANHPFLFIMKHNPTESILFMGRVTNPDTQEIKGRDLDSL
Gene ID
Swiss Prot
SynonymsMEPI; PI14; PANCPIN; TSA2004; SERPINI2
Calculated MW46kDa
Observed MW46kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse pancreas, Rat pancreas
Cellular locationSecreted

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ABclonal:Western blot - SERPINI2 Rabbit pAb (A11732)}

Western blot - SERPINI2 Rabbit pAb (A11732)

Western blot analysis of various lysates using SERPINI2 Rabbit pAb (A11732) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - SERPINI2 Rabbit pAb (A11732)}

Immunofluorescence - SERPINI2 Rabbit pAb (A11732)

Immunofluorescence analysis of paraffin-embedded rat pancreas using SERPINI2 Rabbit pAb (A11732) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SERPINI2 Rabbit pAb (A11732)}

Immunofluorescence - SERPINI2 Rabbit pAb (A11732)

Immunofluorescence analysis of paraffin-embedded mouse pancreas using SERPINI2 Rabbit pAb (A11732) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.