제품 > 항체 > 다중클론항체(pAb)

SETD3 Rabbit pAb (A21681)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - SETD3 Rabbit pAb (A21681)

Western blot analysis of lysates from Jurkat cells, using SETD3 Rabbit pAb (A21681) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 45s.

ABclonal:Immunoprecipitation - SETD3 Rabbit pAb (A21681)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg SETD3 antibody (A21681). Western blot was performed from the immunoprecipitate using SETD3 antibody (A21681) at a dilution of 1:1000.

Overview

Product nameSETD3 Rabbit pAb
Catalog No.A21681
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables protein-L-histidine N-tele-methyltransferase activity. Involved in actin modification and peptidyl-histidine methylation. Located in cytoplasm.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 72-329 of human SETD3 (NP_115609.2).
SequenceDGKREDYFPDLMKWASENGASVEGFEMVNFKEEGFGLRATRDIKAEELFLWVPRKLLMTVESAKNSVLGPLYSQDRILQAMGNIALAFHLLCERASPNSFWQPYIQTLPSEYDTPLYFEEDEVRYLQSTQAIHDVFSQYKNTARQYAYFYKVIQTHPHANKLPLKDSFTYEDYRWAVSSVMTRQNQIPTEDGSRVTLALIPLWDMCNHTNGLITTGYNLEDDRCECVALQDFRAGEQIYIFYGTRSNAEFVIHSGFFF
Gene ID
Swiss Prot
SynonymshSETD3; C14orf154; SETD3
Calculated MW67kDa
Observed MW67kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesJurkat
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

Documents

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ABclonal:Western blot - SETD3 Rabbit pAb (A21681)}

Western blot - SETD3 Rabbit pAb (A21681)

Western blot analysis of lysates from Jurkat cells, using SETD3 Rabbit pAb (A21681) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 45s.
ABclonal:Immunoprecipitation - SETD3 Rabbit pAb (A21681)}

Immunoprecipitation - SETD3 Rabbit pAb (A21681)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg SETD3 antibody (A21681). Western blot was performed from the immunoprecipitate using SETD3 antibody (A21681) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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