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SF3B1 Rabbit mAb (A9737)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

Overview

Product nameSF3B1 Rabbit mAb
Catalog No.A9737
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1724
This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human SF3B1 (O75533).
SequenceMAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQ
Gene ID
Swiss Prot
SynonymsMDS; PRP10; Hsh155; PRPF10; SAP155; SF3b155; SF3B1
Calculated MW146kDa
Observed MW
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, NIH/3T3, Jurkat, Mouse brain
Cellular locationB-WICH complex, Nuclear speck, Nucleolus, Nucleoplasm, Nucleus
Customer validation

IP(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (24)