제품 > 항체 > 다중클론항체(pAb)

SF3B1 Rabbit pAb (A15801)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using SF3B1 Rabbit pAb (A15801) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - SF3B1 Rabbit pAb (A15801)

Immunofluorescence analysis of C6 using SF3B1 Rabbit pAb (A15801) at dilution of d100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 using SF3B1 Rabbit pAb (A15801) at dilution of d100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2OS using SF3B1 Rabbit pAb (A15801) at dilution of d100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name	SF3B1 Rabbit pAb
Catalog No.	A15801
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human SF3B1 (NP_001005526.1).
Sequence	MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADREDEYKKHRRTMIISPERLDPFADGFYSAA
Gene ID
Swiss Prot
Synonyms	MDS; PRP10; Hsh155; PRPF10; SAP155; SF3b155; SF3B1
Calculated MW	146kDa
Observed MW	150kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	Jurkat, HeLa, NIH/3T3, Mouse brain, Mouse liver
Cellular location	Nucleus speckle.
Customer validation	WB(Homo sapiens) IP(Homo sapiens)