제품 > 항체 > 다중클론항체(pAb)

SF3B3/SAP130 Rabbit pAb (A6146)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SF3B3/SAP130 Rabbit pAb (A6146)

Western blot analysis of various lysates using SF3B3/SAP130 Rabbit pAb (A6146) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).

ABclonal:Immunohistochemistry - SF3B3/SAP130 Rabbit pAb (A6146)

Immunohistochemistry analysis of paraffin-embedded Rat brain using SF3B3/SAP130 Rabbit pAb (A6146) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SF3B3/SAP130 Rabbit pAb (A6146)

Immunohistochemistry analysis of paraffin-embedded Human prostate using SF3B3/SAP130 Rabbit pAb (A6146) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SF3B3/SAP130 Rabbit pAb (A6146)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using SF3B3/SAP130 Rabbit pAb (A6146) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameSF3B3/SAP130 Rabbit pAb
Catalog No.A6146
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 948-1217 of human SF3B3/SAP130 (NP_036558.3).
SequenceVPAAIAPFQGRVLIGVGKLLRVYDLGKKKLLRKCENKHIANYISGIQTIGHRVIVSDVQESFIWVRYKRNENQLIIFADDTYPRWVTTASLLDYDTVAGADKFGNICVVRLPPNTNDEVDEDPTGNKALWDRGLLNGASQKAEVIMNYHVGETVLSLQKTTLIPGGSESLVYTTLSGGIGILVPFTSHEDHDFFQHVEMHLRSEHPPLCGRDHLSFRSYYFPVKNVIDGDLCEQFNSMEPNKQKNVSEELDRTPPEVSKKLEDIRTRYAF
Gene ID
Swiss Prot
SynonymsRSE1; SAP130; SF3b130; STAF130; SF3B3/SAP130
Calculated MW136kDa
Observed MW136kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse ovary, Mouse lung
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

IP(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - SF3B3/SAP130 Rabbit pAb (A6146)}

Western blot - SF3B3/SAP130 Rabbit pAb (A6146)

Western blot analysis of various lysates using SF3B3/SAP130 Rabbit pAb (A6146) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
ABclonal:Immunohistochemistry - SF3B3/SAP130 Rabbit pAb (A6146)}

Immunohistochemistry - SF3B3/SAP130 Rabbit pAb (A6146)

Immunohistochemistry analysis of paraffin-embedded Rat brain using SF3B3/SAP130 Rabbit pAb (A6146) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - SF3B3/SAP130 Rabbit pAb (A6146)}

Immunohistochemistry - SF3B3/SAP130 Rabbit pAb (A6146)

Immunohistochemistry analysis of paraffin-embedded Human prostate using SF3B3/SAP130 Rabbit pAb (A6146) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - SF3B3/SAP130 Rabbit pAb (A6146)}

Immunohistochemistry - SF3B3/SAP130 Rabbit pAb (A6146)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using SF3B3/SAP130 Rabbit pAb (A6146) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A6146? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)