제품 > 항체 > 다중클론항체(pAb)

SF3B5 Rabbit pAb (A15905)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SF3B5 Rabbit pAb (A15905)

Western blot analysis of various lysates using SF3B5 Rabbit pAb (A15905) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product nameSF3B5 Rabbit pAb
Catalog No.A15905
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables RNA binding activity and splicing factor binding activity. Involved in mRNA splicing, via spliceosome. Located in nucleoplasm. Part of U12-type spliceosomal complex and U2-type precatalytic spliceosome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-86 of human SF3B5 (NP_112577.1).
SequenceMTDRYTIHSQLEHLQSKYIGTGHADTTKWEWLVNQHRDSYCSYMGHFDLLNYFAIAENESKARVRFNLMEKMLQPCGPPADKPEEN
Gene ID
Swiss Prot
SynonymsYsf3; SF3b10; SF3B5
Calculated MW10kDa
Observed MW11kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, SH-SY5Y, Mouse lung, Rat spleen
Cellular locationNucleus
    ABclonal:Western blot - SF3B5 Rabbit pAb (A15905)}

    Western blot - SF3B5 Rabbit pAb (A15905)

    Western blot analysis of various lysates using SF3B5 Rabbit pAb (A15905) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.