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SGCB Rabbit pAb (A6979)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SGCB Rabbit pAb (A6979)

Western blot analysis of extracts of various cell lines, using SGCB antibody (A6979) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameSGCB Rabbit pAb
Catalog No.A6979
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the sarcoglycan family. Sarcoglycans are transmembrane components in the dystrophin-glycoprotein complex which help stabilize the muscle fiber membranes and link the muscle cytoskeleton to the extracellular matrix. Mutations in this gene have been associated with limb-girdle muscular dystrophy.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 94-318 of human SGCB (NP_000223.1).
SequencePNGCDSMEFHESGLLRFKQVSDMGVIHPLYKSTVGGRRNENLVITGNNQPIVFQQGTTKLSVENNKTSITSDIGMQFFDPRTQNILFSTDYETHEFHLPSGVKSLNVQKASTERITSNATSDLNIKVDGRAIVRGNEGVFIMGKTIEFHMGGNMELKAENSIILNGSVMVSTTRLPSSSSGDQLGSGDWVRYKLCMCADGTLFKVQVTSQNMGCQISDNPCGNTH
Gene ID
Swiss Prot
SynonymsA3b; SGC; LGMD2E; LGMDR4
Calculated MW35kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSKOV3, Mouse heart, Mouse skeletal muscle, Rat heart
Cellular locationCell membrane, Cytoplasm, Single-pass type II membrane protein, cytoskeleton, sarcolemma
Customer validation

WB(Homo sapiens, Rattus norvegicus)

Documents

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    ABclonal:Western blot - SGCB Rabbit pAb (A6979)}

    Western blot - SGCB Rabbit pAb (A6979)

    Western blot analysis of extracts of various cell lines, using SGCB antibody (A6979) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A6979? Please let us know so that we can cite the reference in this datasheet.