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SIAH1 Rabbit mAb (A23247)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SIAH1 Rabbit mAb (A23247)

Western blot analysis of various lysates, using SIAH1 Rabbit mAb (A23247) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - SIAH1 Rabbit mAb (A23247)

Western blot analysis of lysates from mouse liver, using SIAH1 Rabbit mAb (A23247) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - SIAH1 Rabbit mAb (A23247)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using SIAH1 Rabbit mAb (A23247) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SIAH1 Rabbit mAb (A23247)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using SIAH1 Rabbit mAb (A23247) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SIAH1 Rabbit mAb (A23247)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using SIAH1 Rabbit mAb (A23247) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SIAH1 Rabbit mAb (A23247)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using SIAH1 Rabbit mAb (A23247) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SIAH1 Rabbit mAb (A23247)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using SIAH1 Rabbit mAb (A23247) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SIAH1 Rabbit mAb (A23247)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using SIAH1 Rabbit mAb (A23247) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - SIAH1 Rabbit mAb (A23247)

Immunofluorescence analysis of HepG2 cells using SIAH1 Rabbit mAb (A23247) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SIAH1 Rabbit mAb (A23247)

Immunofluorescence analysis of paraffin-embedded human lung using SIAH1 Rabbit mAb (A23247) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameSIAH1 Rabbit mAb
Catalog No.A23247
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC59737
This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human SIAH1. (NP_003022.3).
SequenceMSRQTATALPTGTSKCPPSQRVPALTGTTASNNDLASLFECPVCFDYVLPPILQCQSGHLVCSNCRPKLTCCPTCRGPLGSIRNLAMEKVANSVLFPCKY
Gene ID
Swiss Prot
SynonymsBURHAS; SIAH1A; SIAH1
Calculated MW31kDa
Observed MW31kDa/35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293F, Mouse liver
Cellular locationCytoplasm, Nucleus

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ABclonal:Western blot - SIAH1 Rabbit mAb (A23247)}

Western blot - SIAH1 Rabbit mAb (A23247)

Western blot analysis of various lysates, using SIAH1 Rabbit mAb (A23247) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - SIAH1 Rabbit mAb (A23247)}

Western blot - SIAH1 Rabbit mAb (A23247)

Western blot analysis of lysates from mouse liver, using SIAH1 Rabbit mAb (A23247) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - SIAH1 Rabbit mAb (A23247)}

Immunohistochemistry - SIAH1 Rabbit mAb (A23247)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using SIAH1 Rabbit mAb (A23247) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SIAH1 Rabbit mAb (A23247)}

Immunohistochemistry - SIAH1 Rabbit mAb (A23247)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using SIAH1 Rabbit mAb (A23247) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SIAH1 Rabbit mAb (A23247)}

Immunohistochemistry - SIAH1 Rabbit mAb (A23247)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using SIAH1 Rabbit mAb (A23247) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SIAH1 Rabbit mAb (A23247)}

Immunohistochemistry - SIAH1 Rabbit mAb (A23247)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using SIAH1 Rabbit mAb (A23247) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SIAH1 Rabbit mAb (A23247)}

Immunohistochemistry - SIAH1 Rabbit mAb (A23247)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using SIAH1 Rabbit mAb (A23247) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SIAH1 Rabbit mAb (A23247)}

Immunohistochemistry - SIAH1 Rabbit mAb (A23247)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using SIAH1 Rabbit mAb (A23247) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - SIAH1 Rabbit mAb (A23247)}

Immunofluorescence - SIAH1 Rabbit mAb (A23247)

Immunofluorescence analysis of HepG2 cells using SIAH1 Rabbit mAb (A23247) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SIAH1 Rabbit mAb (A23247)}

Immunofluorescence - SIAH1 Rabbit mAb (A23247)

Immunofluorescence analysis of paraffin-embedded human lung using SIAH1 Rabbit mAb (A23247) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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