제품 > 항체 > 다중클론항체(pAb)

SIX2 Rabbit pAb (A8727)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - SIX2 Rabbit pAb (A8727)

Western blot analysis of various lysates using SIX2 Rabbit pAb (A8727) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - SIX2 Rabbit pAb (A8727)

Western blot analysis of lysates from Rat skeletal muscle , using SIX2 Rabbit pAb (A8727) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameSIX2 Rabbit pAb
Catalog No.A8727
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the vertebrate gene family which encode proteins homologous to the Drosophila 'sine oculis' homeobox protein. The encoded protein is a transcription factor which, like other members of this gene family, may be involved in limb or eye development.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 120-174 of human SIX2 (NP_058628.3).
SequenceSIWDGEETSYCFKEKSRSVLREWYAHNPYPSPREKRELAEATGLTTTQVSNWFKN
Gene ID
Swiss Prot
SynonymsSIX2
Calculated MW32kDa
Observed MW32kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, HepG2, RD, Rat skeletal muscle
Cellular locationNucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - SIX2 Rabbit pAb (A8727)}

    Western blot - SIX2 Rabbit pAb (A8727)

    Western blot analysis of various lysates using SIX2 Rabbit pAb (A8727) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - SIX2 Rabbit pAb (A8727)}

    Western blot - SIX2 Rabbit pAb (A8727)

    Western blot analysis of lysates from Rat skeletal muscle , using SIX2 Rabbit pAb (A8727) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)