제품 > 항체 > 다중클론항체(pAb)

SLC15A1 Rabbit pAb (A10246)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - SLC15A1 Rabbit pAb (A10246)

Western blot analysis of extracts of various cell lines, using SLC15A1 antibody (A10246) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product nameSLC15A1 Rabbit pAb
Catalog No.A10246
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an intestinal hydrogen peptide cotransporter that is a member of the solute carrier family 15. The encoded protein is localized to the brush border membrane of the intestinal epithelium and mediates the uptake of di- and tripeptides from the lumen into the enterocytes. This protein plays an important role in the uptake and digestion of dietary proteins. This protein also facilitates the absorption of numerous peptidomimetic drugs.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 600-700 of human SLC15A1 (NP_005064.1).
SequenceVTGLEFSYSQAPSNMKSVLQAGWLLTVAVGNIIVLIVAGAGQFSKQWAEYILFAALLLVVCVIFAIMARFYTYINPAEIEAQFDEDEKKNRLEKSNPYFMS
Gene ID
Swiss Prot
SynonymsPEPT1; HPECT1; HPEPT1
Calculated MW79kDa
Observed MW78kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse lung, Rat testis
Cellular locationMembrane, Multi-pass membrane protein
    ABclonal:Western blot - SLC15A1 Rabbit pAb (A10246)}

    Western blot - SLC15A1 Rabbit pAb (A10246)

    Western blot analysis of extracts of various cell lines, using SLC15A1 antibody (A10246) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.