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SLC25A13/Citrin Rabbit mAb (A23756)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SLC25A13/Citrin Rabbit mAb (A23756)

Western blot analysis of various lysates, using SLC25A13/Citrin Rabbit mAb (A23756) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): MOLT-4
Exposure time: 10s.

ABclonal:Western blot - SLC25A13/Citrin Rabbit mAb (A23756)

Western blot analysis of various lysates, using SLC25A13/Citrin Rabbit mAb (A23756) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

ABclonal:Immunohistochemistry - SLC25A13/Citrin Rabbit mAb (A23756)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using SLC25A13/Citrin Rabbit mAb (A23756) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SLC25A13/Citrin Rabbit mAb (A23756)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using SLC25A13/Citrin Rabbit mAb (A23756) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SLC25A13/Citrin Rabbit mAb (A23756)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using SLC25A13/Citrin Rabbit mAb (A23756) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SLC25A13/Citrin Rabbit mAb (A23756)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using SLC25A13/Citrin Rabbit mAb (A23756) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SLC25A13/Citrin Rabbit mAb (A23756)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using SLC25A13/Citrin Rabbit mAb (A23756) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SLC25A13/Citrin Rabbit mAb (A23756)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using SLC25A13/Citrin Rabbit mAb (A23756) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameSLC25A13/Citrin Rabbit mAb
Catalog No.A23756
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC61350
This gene is a member of the mitochondrial carrier family. The encoded protein contains four EF-hand Ca(2+) binding motifs in the N-terminal domain, and localizes to mitochondria. The protein catalyzes the exchange of aspartate for glutamate and a proton across the inner mitochondrial membrane, and is stimulated by calcium on the external side of the inner mitochondrial membrane. Mutations in this gene result in citrullinemia, type II. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SLC25A13/Citrin. (NP_055066.1).
SequenceMAAAKVALTKRADPAELRTIFLKYASIEKNGEFFMSPNDFVTRYLNIFGESQPNPKTVELLSGVVDQTKDGLISFQEFVAFESVLCAPDALFMVAFQLFDKAGKGEVTFEDVKQVFGQTTIHQHIPFNWDSEFVQLHFGKERKRHLTYAEFTQFLLEIQLEHAKQAFVQRDNARTGRVTAIDFRDIMVTIRPHVLTPFVEECLVAAAGGTTSHQVSFSYFNGFNSLLNNMELIRKIYSTLAGTRKDVEVTKEEFVLAAQKFGQVTPMEVDILFQLADLYEPRGRMTLADIERIAPLEEGT
Gene ID
Swiss Prot
SynonymsSLC25A13; ARALAR2; CITRIN; CTLN2; solute carrier family 25 member 13; SLC25A13/Citrin
Calculated MW74kDa
Observed MW74kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples A-431, Hep G2, Mouse liver, Mouse kidney, Rat liver
Cellular locationMitochondrion inner membrane, Multi-pass membrane protein

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - SLC25A13/Citrin Rabbit mAb (A23756)}

    Western blot - SLC25A13/Citrin Rabbit mAb (A23756)

    Western blot analysis of various lysates, using SLC25A13/Citrin Rabbit mAb (A23756) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Negative control (NC): MOLT-4
    Exposure time: 10s.
    ABclonal:Western blot - SLC25A13/Citrin Rabbit mAb (A23756)}

    Western blot - SLC25A13/Citrin Rabbit mAb (A23756)

    Western blot analysis of various lysates, using SLC25A13/Citrin Rabbit mAb (A23756) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 0.5s.
    ABclonal:Immunohistochemistry - SLC25A13/Citrin Rabbit mAb (A23756)}

    Immunohistochemistry - SLC25A13/Citrin Rabbit mAb (A23756)

    Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using SLC25A13/Citrin Rabbit mAb (A23756) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - SLC25A13/Citrin Rabbit mAb (A23756)}

    Immunohistochemistry - SLC25A13/Citrin Rabbit mAb (A23756)

    Immunohistochemistry analysis of paraffin-embedded Human liver tissue using SLC25A13/Citrin Rabbit mAb (A23756) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - SLC25A13/Citrin Rabbit mAb (A23756)}

    Immunohistochemistry - SLC25A13/Citrin Rabbit mAb (A23756)

    Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using SLC25A13/Citrin Rabbit mAb (A23756) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - SLC25A13/Citrin Rabbit mAb (A23756)}

    Immunohistochemistry - SLC25A13/Citrin Rabbit mAb (A23756)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using SLC25A13/Citrin Rabbit mAb (A23756) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - SLC25A13/Citrin Rabbit mAb (A23756)}

    Immunohistochemistry - SLC25A13/Citrin Rabbit mAb (A23756)

    Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using SLC25A13/Citrin Rabbit mAb (A23756) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - SLC25A13/Citrin Rabbit mAb (A23756)}

    Immunohistochemistry - SLC25A13/Citrin Rabbit mAb (A23756)

    Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using SLC25A13/Citrin Rabbit mAb (A23756) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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