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SMC4 Rabbit mAb (A3559)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SMC4 Rabbit mAb (A3559)

Western blot analysis of extracts of various cell lines, using SMC4 Rabbit mAb (A3559) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - SMC4 Rabbit mAb (A3559)

Immunofluorescence analysis of C6 cells using SMC4 Rabbit mAb (A3559) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SMC4 Rabbit mAb (A3559)

Immunofluorescence analysis of NIH/3T3 cells using SMC4 Rabbit mAb (A3559) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SMC4 Rabbit mAb (A3559)

Immunofluorescence analysis of U-2 OS cells using SMC4 Rabbit mAb (A3559) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameSMC4 Rabbit mAb
Catalog No.A3559
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2042
This gene belongs to the 'structural maintenance of chromosomes' (SMC) gene family. Members of this gene family play a role in two changes in chromosome structure during mitotic segregation of chromosomes- chromosome condensation and sister chromatid cohesion. The protein encoded by this gene is likely a subunit of the 13S condensin complex, which is involved in chromosome condensation. A pseudogene related to this gene is located on chromosome 2.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1189-1288 of human SMC4 (Q9NTJ3).
SequenceFNLSGGEKTLSSLALVFALHHYKPTPLYFMDEIDAALDFKNVSIVAFYIYEQTKNAQFIIISLRNNMFEISDRLIGIYKTYNITKSVAVNPKEIASKGLC
Gene ID
Swiss Prot
SynonymsCAPC; CAP-C; SMC-4; SMC4L1
Calculated MW147kDa
Observed MW180kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHT-29, NIH/3T3, Raji, C6, Mouse testis, Mouse thymus, Rat testis
Cellular locationChromosome, Cytoplasm, Nucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - SMC4 Rabbit mAb (A3559)}

    Western blot - SMC4 Rabbit mAb (A3559)

    Western blot analysis of extracts of various cell lines, using SMC4 Rabbit mAb (A3559) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunofluorescence - SMC4 Rabbit mAb (A3559)}

    Immunofluorescence - SMC4 Rabbit mAb (A3559)

    Immunofluorescence analysis of C6 cells using SMC4 Rabbit mAb (A3559) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - SMC4 Rabbit mAb (A3559)}

    Immunofluorescence - SMC4 Rabbit mAb (A3559)

    Immunofluorescence analysis of NIH/3T3 cells using SMC4 Rabbit mAb (A3559) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - SMC4 Rabbit mAb (A3559)}

    Immunofluorescence - SMC4 Rabbit mAb (A3559)

    Immunofluorescence analysis of U-2 OS cells using SMC4 Rabbit mAb (A3559) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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