제품 > 항체 > 다중클론항체(pAb)

SMEK1 Rabbit pAb (A8500)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SMEK1 Rabbit pAb (A8500)

Western blot analysis of various lysates using SMEK1 Rabbit pAb (A8500) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - SMEK1 Rabbit pAb (A8500)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using SMEK1 Rabbit pAb (A8500) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SMEK1 Rabbit pAb (A8500)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using SMEK1 Rabbit pAb (A8500) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SMEK1 Rabbit pAb (A8500)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using SMEK1 Rabbit pAb (A8500) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameSMEK1 Rabbit pAb
Catalog No.A8500
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to act upstream of or within positive regulation of gluconeogenesis and protein dephosphorylation. Located in cytosol and nuclear speck.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 395-594 of human SMEK1 (NP_001271210.1).
SequenceVQTFKGLKLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPISKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGSVPKNTSQTAAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPLSKKAKFDS
Gene ID
Swiss Prot
Synonymssmk1; FLFL1; PP4R3; SMEK1; smk-1; PP4R3A; MSTP033; KIAA2010
Calculated MW95kDa
Observed MW110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, HepG2, Jurkat, MCF-7, Mouse spleen, Rat brain
Cellular locationCytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Customer validation

IF(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - SMEK1 Rabbit pAb (A8500)}

    Western blot - SMEK1 Rabbit pAb (A8500)

    Western blot analysis of various lysates using SMEK1 Rabbit pAb (A8500) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - SMEK1 Rabbit pAb (A8500)}

    Immunohistochemistry - SMEK1 Rabbit pAb (A8500)

    Immunohistochemistry analysis of paraffin-embedded Rat ovary using SMEK1 Rabbit pAb (A8500) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - SMEK1 Rabbit pAb (A8500)}

    Immunohistochemistry - SMEK1 Rabbit pAb (A8500)

    Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using SMEK1 Rabbit pAb (A8500) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - SMEK1 Rabbit pAb (A8500)}

    Immunohistochemistry - SMEK1 Rabbit pAb (A8500)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney using SMEK1 Rabbit pAb (A8500) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A8500? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)