제품 > 항체 > 다중클론항체(pAb)

SMPD1 / ASM Rabbit pAb (A6743)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using SMPD1 / ASM Rabbit pAb (A6743) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 5s.

ABclonal:Immunofluorescence - SMPD1 / ASM Rabbit pAb (A6743)

Immunofluorescence analysis of HeLa cells using SMPD1 / ASM Rabbit pAb (A6743). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	SMPD1 / ASM Rabbit pAb
Catalog No.	A6743
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 70-340 of human SMPD1 / ASM (NP_000534.3).
Sequence	PARLHRIVPRLRDVFGWGNLTCPICKGLFTAINLGLKKEPNVARVGSVAIKLCNLLKIAPPAVCQSIVHLFEDDMVEVWRRSVLSPSEACGLLLGSTCGHWDIFSSWNISLPTVPKPPPKPPSPPAPGAPVSRILFLTDLHWDHDYLEGTDPDCADPLCCRRGSGLPPASRPGAGYWGEYSKCDLPLRTLESLLSGLGPAGPFDMVYWTGDIPAHDVWHQTRQDQLRALTTVTALVRKFLGPVPVYPAVGNHESTPVNSFPPPFIEGNHSS
Gene ID
Swiss Prot
Synonyms	ASM; NPD; ASMASE; SMPD1 / ASM
Calculated MW	70kDa
Observed MW	55kDa/70kDa/60kDa, 70-75kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500 IF/ICC 1:10 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HepG2, MCF7, Hep G2, U-251 MG, MCF7, Mouse liver, Rat brain
Cellular location	Lysosome
Customer validation	WB(Homo sapiens, Rattus norvegicus, Mus musculus) IHC(Mus musculus)

Documents

Certificate of Compliance

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Research Area

SMPD1 / ASM Rabbit pAb images

Western blot - SMPD1 / ASM Rabbit pAb (A6743)

Immunofluorescence - SMPD1 / ASM Rabbit pAb (A6743)

Immunofluorescence analysis of HeLa cells using SMPD1 / ASM Rabbit pAb (A6743). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.