제품 > 항체 > 다중클론항체(pAb)

SMPD4 Rabbit pAb (A15473)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - SMPD4 Rabbit pAb (A15473)

Western blot analysis of lysates from A-549 cells, using SMPD4 Rabbit pAb (A15473) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

ABclonal:Immunofluorescence - SMPD4 Rabbit pAb (A15473)

Immunofluorescence analysis of L929 cells using SMPD4 Rabbit pAb (A15473) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameSMPD4 Rabbit pAb
Catalog No.A15473
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a sphingomyelinase that catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. This gene is activated by DNA damage, cellular stress, and tumor necrosis factor, but it is downregulated by wild-type p53. The encoded protein localizes to the endoplasmic reticulum and Golgi network.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 600-800 of human SMPD4 (NP_060421.2).
SequenceITQAKHTAKSISDQCAESPAGHSFLSWLGFSSMDTNGSYTANDLDEMGQDSVRKTDEYLEKALEYLRQIFRLSEAQLRQFTLALGTTQDENGKKQLPDCIVGEDGLILTPLGRYQIINGLRRFEIEYQGDPELQPIRSYEIASLVRTLFRLSSAINHRFAGQMAALCSRDDFLGSFCRYHLTEPGLASRHLLSPVGRRQVA
Gene ID
Swiss Prot
SynonymsSKNY; NET13; NEDMABA; NEDMEBA; NSMASE3; NSMASE-3; SMPD4
Calculated MW98kDa
Observed MW100kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesA-549
Cellular locationEndoplasmic reticulum membrane, Golgi apparatus membrane, Single-pass membrane protein

Documents

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    ABclonal:Western blot - SMPD4 Rabbit pAb (A15473)}

    Western blot - SMPD4 Rabbit pAb (A15473)

    Western blot analysis of lysates from A-549 cells, using SMPD4 Rabbit pAb (A15473) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 15s.
    ABclonal:Immunofluorescence - SMPD4 Rabbit pAb (A15473)}

    Immunofluorescence - SMPD4 Rabbit pAb (A15473)

    Immunofluorescence analysis of L929 cells using SMPD4 Rabbit pAb (A15473) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.