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SMYD4 Rabbit pAb (A7310)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SMYD4 Rabbit pAb (A7310)

Western blot analysis of lysates from HeLa cells, using SMYD4 Rabbit pAb (A7310) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - SMYD4 Rabbit pAb (A7310)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using SMYD4 Rabbit pAb (A7310) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SMYD4 Rabbit pAb (A7310)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using SMYD4 Rabbit pAb (A7310) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SMYD4 Rabbit pAb (A7310)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using SMYD4 Rabbit pAb (A7310) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - SMYD4 Rabbit pAb (A7310)

Immunofluorescence analysis of A-549 cells using SMYD4 Rabbit pAb (A7310). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - SMYD4 Rabbit pAb (A7310)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug SMYD4 Rabbit pAb (A7310 1:2000). Western blot was performed from the immunoprecipitate using SMYD4 Rabbit pAb (A7310) at a dilition of 1:1000.

Overview

Product nameSMYD4 Rabbit pAb
Catalog No.A7310
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable metal ion binding activity and methyltransferase activity. Involved in heart development. Predicted to be located in cytoplasm and nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SMYD4 (NP_443160.2).
SequenceMDLPVDEWKSYLLQKWASLPTSVQVTISTAETLRDIFLHSSSLLQPEDELFLKRLSKGYLVGKDSDAPLFYREEGNKKFQEKDYTGAAVLYSKGVSHSRPNTEDMSLCHANRSAALFHLGQYETCLKDINRAQTHGYPERLQPKIMLRKAECLVALGRLQEASQTISDLERNFTATPALADVLPQTLQRNLHRLKMKMQEKDSLTESFPAALAKTLEDAALREENEQLSNASSSIGLCVDPLKGRCLVATKDILPGELLV
Gene ID
Swiss Prot
SynonymsZMYND21; SMYD4
Calculated MW89kDa
Observed MW89kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:100
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa
Cellular locationcytoplasm, nucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - SMYD4 Rabbit pAb (A7310)}

    Western blot - SMYD4 Rabbit pAb (A7310)

    Western blot analysis of lysates from HeLa cells, using SMYD4 Rabbit pAb (A7310) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunohistochemistry - SMYD4 Rabbit pAb (A7310)}

    Immunohistochemistry - SMYD4 Rabbit pAb (A7310)

    Immunohistochemistry analysis of paraffin-embedded Rat spleen using SMYD4 Rabbit pAb (A7310) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - SMYD4 Rabbit pAb (A7310)}

    Immunohistochemistry - SMYD4 Rabbit pAb (A7310)

    Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using SMYD4 Rabbit pAb (A7310) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - SMYD4 Rabbit pAb (A7310)}

    Immunohistochemistry - SMYD4 Rabbit pAb (A7310)

    Immunohistochemistry analysis of paraffin-embedded Mouse lung using SMYD4 Rabbit pAb (A7310) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - SMYD4 Rabbit pAb (A7310)}

    Immunofluorescence - SMYD4 Rabbit pAb (A7310)

    Immunofluorescence analysis of A-549 cells using SMYD4 Rabbit pAb (A7310). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunoprecipitation - SMYD4 Rabbit pAb (A7310)}

    Immunoprecipitation - SMYD4 Rabbit pAb (A7310)

    Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug SMYD4 Rabbit pAb (A7310 1:2000). Western blot was performed from the immunoprecipitate using SMYD4 Rabbit pAb (A7310) at a dilition of 1:1000.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)