제품 > 항체 > 다중클론항체(pAb)

SOX14 Rabbit pAb (A13674)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SOX14 Rabbit pAb (A13674)

Western blot analysis of lysates from HepG2 cells, using SOX14 Rabbit pAb (A13674) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - SOX14 Rabbit pAb (A13674)

Immunofluorescence analysis of MCF-7 cells using SOX14 Rabbit pAb (A13674).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

Overview

Product nameSOX14 Rabbit pAb
Catalog No.A13674
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. Mutations in this gene are suggested to be responsible for the limb defects associated with blepharophimosis, ptosis, epicanthus inversus syndrome (BPES) and Mobius syndrome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 91-240 of human SOX14 (NP_004180.1).
SequenceRYVFPLPYLGDTDPLKAAGLPVGASDGLLSAPEKARAFLPPASAPYSLLDPAQFSSSAIQKMGEVPHTLATGALPYASTLGYQNGAFGSLSCPSQHTHTHPSPTNPGYVVPCNCTAWSASTLQPPVAYILFPGMTKTGIDPYSSAHATAM
Gene ID
Swiss Prot
SynonymsSOX28; SOX14
Calculated MW26kDa
Observed MW24kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHepG2
Cellular locationNucleus

Documents

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ABclonal:Western blot - SOX14 Rabbit pAb (A13674)}

Western blot - SOX14 Rabbit pAb (A13674)

Western blot analysis of lysates from HepG2 cells, using SOX14 Rabbit pAb (A13674) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - SOX14 Rabbit pAb (A13674)}

Immunofluorescence - SOX14 Rabbit pAb (A13674)

Immunofluorescence analysis of MCF-7 cells using SOX14 Rabbit pAb (A13674).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

* For research use only. Not for therapeutic or diagnostic purposes.