Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina(RK20265) from 10⁵ Hep G2 cells with 1 μg SP1 Rabbit mAb (A19649), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of SP1 in representative gene loci (TINF2), as shown in figure.
Chromatin immunoprecipitation was performed with 25 μg of cross-linked chromatin from 293T cells using 5 μg of SP1 Rabbit mAb (A19649). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of SP1 across chromosome 14 (upper panel) and the genomic region encompassing TINF2, a representative gene enriched in SP1 (lower panel).
Chromatin immunoprecipitation was performed with 25 μg of cross-linked chromatin from 293T cells using 5 μg of SP1 Rabbit mAb (A19649). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of SP1 in the representative genomic region surrounding TINF2 gene.
Western blot analysis of various lysates using SP1 Rabbit mAb (A19649) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using SP1 Rabbit mAb (A19649) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using SP1 Rabbit mAb (A19649) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using SP1 Rabbit mAb (A19649) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using SP1 Rabbit mAb (A19649) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using SP1 Rabbit mAb (A19649) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using SP1 Rabbit mAb (A19649) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of NIH-3T3 cells using SP1 Rabbit mAb (A19649) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Confocal imaging of C6 cells using SP1 Rabbit mAb (A19649, at dilution of 1:100) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
| Product name | SP1 Rabbit mAb |
|---|---|
| Catalog No. | A19649 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC0128 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 500-600 of human SP1 (P08047). |
|---|---|
| Sequence | TTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGTSGIQVHPIQGLPLAIANAPGDHGAQLGLHGAGGDGIHDDTAGGEEGENSPDAQPQAGRRTR |
| Gene ID | |
| Swiss Prot | |
| Synonyms | SP1 |
| Calculated MW | 81kDa |
| Observed MW | 90kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | HeLa, U-87MG, Rat liver |
| Cellular location | Cytoplasm, Nucleus. |
| Customer validation | ChIP(Homo sapiens, Mus musculus) qPCR(Homo sapiens) Other(Homo sapiens) WB(Mus musculus, Homo sapiens) ChIP(Mus musculus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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