제품 > 항체 > 다중클론항체(pAb)

SPEM1 Rabbit pAb (A8076)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - SPEM1 Rabbit pAb (A8076)

Western blot analysis of extracts of PC-3 cells, using SPEM1 antibody (A8076) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameSPEM1 Rabbit pAb
Catalog No.A8076
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to be involved in flagellated sperm motility and sperm individualization. Predicted to act upstream of or within spermatogenesis. Predicted to be integral component of membrane. Predicted to be active in cytoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 203-309 of human SPEM1 (NP_955371.2).
SequenceYVYPVNPPPPSPEAPSHKNGGEGAVPEAEAAQYQPVPAPTLGPAVIPEFSRHRSSGRIVYDARDMRRRLRELTREVEALSGCYPLASGSSTAEETSKNWVYRSLTGR
Gene ID
Swiss Prot
SynonymsC17orf83
Calculated MW35kDa
Observed MW36kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesPC-3
Cellular locationCytoplasm, Membrane, Single-pass membrane protein
    ABclonal:Western blot - SPEM1 Rabbit pAb (A8076)}

    Western blot - SPEM1 Rabbit pAb (A8076)

    Western blot analysis of extracts of PC-3 cells, using SPEM1 antibody (A8076) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)