제품 > 항체 > 단일클론항체(mAb)

SPINK5 / LEKTI Rabbit mAb (A20916)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SPINK5 / LEKTI Rabbit mAb (A20916)

Western blot analysis of various lysates using SPINK5 / LEKTI Rabbit mAb (A20916) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameSPINK5 / LEKTI Rabbit mAb
Catalog No.A20916
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2888
This gene encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may exhibit unique activities and specificities. These proteins may play a role in skin and hair morphogenesis, as well as anti-inflammatory and antimicrobial protection of mucous epithelia. Mutations in this gene may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. This gene is present in a gene cluster on chromosome 5. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human SPINK5 / LEKTI (Q9NQ38).
SequenceFKKGERDGDFICPDYYEAVCGTDGKTYDNRCALCAENAKTGSQIGVKSEGECKSSNPEQDVCSAFRPFVRDGRLGCTRENDPVLGPDGKTHGNKCAMCAEL
Gene ID
Swiss Prot
SynonymsNS; NETS; LEKTI; LETKI; VAKTI; SPINK5 / LEKTI
Calculated MW121kDa
Observed MW37kDa/65kDa/145kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, MCF7, RAW264.7, Mouse skin, Mouse stomach, Rat stomach
Cellular locationSecreted

Documents

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    ABclonal:Western blot - SPINK5 / LEKTI Rabbit mAb (A20916)}

    Western blot - SPINK5 / LEKTI Rabbit mAb (A20916)

    Western blot analysis of various lysates using SPINK5 / LEKTI Rabbit mAb (A20916) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)