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SPON2 Rabbit pAb (A12077)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - SPON2 Rabbit pAb (A12077)

Western blot analysis of various lysates using SPON2 Rabbit pAb (A12077) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - SPON2 Rabbit pAb (A12077)

Immunohistochemistry analysis of paraffin-embedded Human stomach using SPON2 Rabbit pAb (A12077) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - SPON2 Rabbit pAb (A12077)

Immunohistochemistry analysis of paraffin-embedded Human placenta using SPON2 Rabbit pAb (A12077) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameSPON2 Rabbit pAb
Catalog No.A12077
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable antigen binding activity; lipopolysaccharide binding activity; and metal ion binding activity. Predicted to be involved in cell adhesion. Predicted to act upstream of or within several processes, including defense response to other organism; opsonization; and positive regulation of cytokine production. Located in extracellular exosome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 27-150 of human SPON2 (NP_036577.1).
SequenceQPLGGESICSARAPAKYSITFTGKWSQTAFPKQYPLFRPPAQWSSLLGAAHSSDYSMWRKNQYVSNGLRDFAERGEAWALMKEIEAAGEALQSVHAVFSAPAVPSGTGQTSAELEVQRRHSLVS
Gene ID
Swiss Prot
SynonymsDIL1; DIL-1; MINDIN; M-SPONDIN; SPON2
Calculated MW36kDa
Observed MW36kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHCT116, HeLa, Rat brain
Cellular locationSecreted, extracellular matrix, extracellular space.

Documents

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    ABclonal:Western blot - SPON2 Rabbit pAb (A12077)}

    Western blot - SPON2 Rabbit pAb (A12077)

    Western blot analysis of various lysates using SPON2 Rabbit pAb (A12077) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - SPON2 Rabbit pAb (A12077)}

    Immunohistochemistry - SPON2 Rabbit pAb (A12077)

    Immunohistochemistry analysis of paraffin-embedded Human stomach using SPON2 Rabbit pAb (A12077) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - SPON2 Rabbit pAb (A12077)}

    Immunohistochemistry - SPON2 Rabbit pAb (A12077)

    Immunohistochemistry analysis of paraffin-embedded Human placenta using SPON2 Rabbit pAb (A12077) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)