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SPOP Rabbit pAb (A12106)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - SPOP Rabbit pAb (A12106)

Western blot analysis of various lysates using SPOP Rabbit pAb (A12106) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product nameSPOP Rabbit pAb
Catalog No.A12106
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 220-374 of human SPOP (NP_001007227.1).
SequenceARSPVFSAMFEHEMEESKKNRVEINDVEPEVFKEMMCFIYTGKAPNLDKMADDLLAAADKYALERLKVMCEDALCSNLSVENAAEILILADLHSADQLKTQAVDFINYHASDVLETSGWKSMVVSHPHLVAEAYRSLASAQCPFLGPPRKRLKQS
Gene ID
Swiss Prot
SynonymsTEF2; BTBD32; NSDVS1; NSDVS2; NEDMACE; NEDMIDF; SPOP
Calculated MW42kDa
Observed MW42kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, HT-29
Cellular locationNucleus, Nucleus speckle

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    ABclonal:Western blot - SPOP Rabbit pAb (A12106)}

    Western blot - SPOP Rabbit pAb (A12106)

    Western blot analysis of various lysates using SPOP Rabbit pAb (A12106) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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