제품 > 항체 > 다중클론항체(pAb)

SPOP Rabbit pAb (A7621)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - SPOP Rabbit pAb (A7621)

Western blot analysis of extracts of various cell lines, using SPOP antibody (A7621) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameSPOP Rabbit pAb
Catalog No.A7621
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables RNA polymerase II-specific DNA-binding transcription factor binding activity. Acts upstream of or within several processes, including glucose homeostasis; positive regulation of apoptotic process; and regulation of transcription, DNA-templated. Located in nuclear speck. Is expressed in lung; metanephros; and pancreas. Orthologous to human SPOP (speckle type BTB/POZ protein).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-270 of mouse SPOP (NP_079563.2).
SequenceMSRVPSPPPPAEMSSGPVAESWCYTQIKVVKFSYMWTINNFSFCREEMGEVIKSSTFSSGANDKLKWCLRVNPKGLDEESKDYLSLYLLLVSCPKSEVRAKFKFSILNAKGEETKAMESQRAYRFVQGKDWGFKKFIRRDFLLDEANGLLPDDKLTLFCEVSVVQDSVNISGQNTMNMVKVPECRLADELGGLWENSRFTDCCLCVAGQEFQAHKAILAARSPVFSAMFEHEMEESKKNRVEINDVEPEVFKEMMCFIYTGKAPNLDKMA
Gene ID
Swiss Prot
SynonymsTEF2; Pcif1
Calculated MW42kDa
Observed MW42kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesBT-474, Mouse pancreas
Cellular locationNucleus, Nucleus speckle
Customer validation

WB(Homo sapiens)

ChIP(Mus musculus)

Pull-down(Mus musculus)

WB(Mus musculus)

    ABclonal:Western blot - SPOP Rabbit pAb (A7621)}

    Western blot - SPOP Rabbit pAb (A7621)

    Western blot analysis of extracts of various cell lines, using SPOP antibody (A7621) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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