제품 > 항체 > 다중클론항체(pAb)

SPRY3 Rabbit pAb (A14669)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - SPRY3 Rabbit pAb (A14669)

Western blot analysis of lysates from 293T cells, using SPRY3 Rabbit pAb (A14669) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameSPRY3 Rabbit pAb
Catalog No.A14669
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Involved in negative regulation of MAPK cascade. Predicted to be located in membrane. Predicted to be active in cytosol.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human SPRY3 (NP_005831.1).
SequenceTDDEDNCADEPCSCGPSSCFVRWAAMSLISLFLPCLCCYLPTRGCLHLCQQGYDSLRRPGCRCKRHTNTVCRKISSGSAPFPKAQEKSV
Gene ID
Swiss Prot
Synonymsspry-3; SPRY3
Calculated MW31kDa
Observed MW36kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T
Cellular locationCytoplasm, Membrane, Peripheral membrane protein
ABclonal:Western blot - SPRY3 Rabbit pAb (A14669)}

Western blot - SPRY3 Rabbit pAb (A14669)

Western blot analysis of lysates from 293T cells, using SPRY3 Rabbit pAb (A14669) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.