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SRD5A2 Rabbit mAb (A19762)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SRD5A2 Rabbit mAb (A19762)

Western blot analysis of various lysates using SRD5A2 Rabbit mAb (A19762) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - SRD5A2 Rabbit mAb (A19762)

Immunofluorescence analysis of paraffin-embedded mouse testis using SRD5A2 Rabbit mAb (A19762) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SRD5A2 Rabbit mAb (A19762)

Immunofluorescence analysis of paraffin-embedded rat testis using SRD5A2 Rabbit mAb (A19762) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameSRD5A2 Rabbit mAb
Catalog No.A19762
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2287
This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human SRD5A2 (P31213).
SequenceMQVQCQQSPVLAGSATLVALGALALYVAKPSGYGKHTESLKPAATRLPARAAWFLQELPSFAVPAGILARQPLSLFGPPGTVLLGLFCVHYFHRTFVYSL
Gene ID
Swiss Prot
Synonyms
Calculated MW28kDa
Observed MW28kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IF/ICC 1:100 - 1:400
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesPC-3, Mouse liver, Mouse testis, Mouse kidney, Rat testis, Rat kidney
Cellular locationcell body fiber.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - SRD5A2 Rabbit mAb (A19762)}

Western blot - SRD5A2 Rabbit mAb (A19762)

Western blot analysis of various lysates using SRD5A2 Rabbit mAb (A19762) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - SRD5A2 Rabbit mAb (A19762)}

Immunofluorescence - SRD5A2 Rabbit mAb (A19762)

Immunofluorescence analysis of paraffin-embedded mouse testis using SRD5A2 Rabbit mAb (A19762) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SRD5A2 Rabbit mAb (A19762)}

Immunofluorescence - SRD5A2 Rabbit mAb (A19762)

Immunofluorescence analysis of paraffin-embedded rat testis using SRD5A2 Rabbit mAb (A19762) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A19762? Please let us know so that we can cite the reference in this datasheet.

ELISA 키트 (1)

Secondary Antibodies (26)