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SRSF6 Rabbit pAb (A14603)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SRSF6 Rabbit pAb (A14603)

Western blot analysis of various lysates using SRSF6 Rabbit pAb (A14603) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - SRSF6 Rabbit pAb (A14603)

Western blot analysis of lysates from Mouse lung, using SRSF6 Rabbit pAb (A14603) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameSRSF6 Rabbit pAb
Catalog No.A14603
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is involved in mRNA splicing and may play a role in the determination of alternative splicing. The encoded nuclear protein belongs to the splicing factor SR family and has been shown to bind with and modulate another member of the family, SFRS12. Alternative splicing results in multiple transcript variants. In addition, two pseudogenes, one on chromosome 17 and the other on the X chromosome, have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human SRSF6 (NP_006266.2).
SequenceMPRVYIGRLSYNVREKDIQRFFSGYGRLLEVDLKNGYGFVEFEDSRDADDAVYELNGKELCGERVIVEHARGPRRDRDGYSYGSRSGGGGYSSRRTSGRD
Gene ID
Swiss Prot
SynonymsB52; SFRS6; SRP55; HEL-S-91; SRSF6
Calculated MW40kDa
Observed MW55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHT-29, K-562, HeLa, HepG2, Mouse lung
Cellular locationNucleus, Nucleus speckle
Customer validation

WB(Mus musculus)

    ABclonal:Western blot - SRSF6 Rabbit pAb (A14603)}

    Western blot - SRSF6 Rabbit pAb (A14603)

    Western blot analysis of various lysates using SRSF6 Rabbit pAb (A14603) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Western blot - SRSF6 Rabbit pAb (A14603)}

    Western blot - SRSF6 Rabbit pAb (A14603)

    Western blot analysis of lysates from Mouse lung, using SRSF6 Rabbit pAb (A14603) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    항체 (1)

    Secondary Antibodies (26)