제품 > 항체 > 다중클론항체(pAb)

ST3GAL1 Rabbit pAb (A11616)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - ST3GAL1 Rabbit pAb (A11616)

Western blot analysis of lysates from mouse liver, using ST3GAL1 Rabbit pAb (A11616) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 40s.

Overview

Product nameST3GAL1 Rabbit pAb
Catalog No.A11616
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. Correct glycosylation of the encoded protein may be critical to its sialyltransferase activity. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4B. Two transcript variants encoding the same protein have been found for this gene. Other transcript variants may exist, but have not been fully characterized yet.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 35-135 of human ST3GAL1 (NP_003024.1).
SequenceTTWFPKQMVLELSENLKRLIKHRPCTCTHCIGQRKLSAWFDERFNQTMQPLLTAQNALLEDDTYRWWLRLQREKKPNNLNDTIKELFRVVPGNVDPMLEKR
Gene ID
Swiss Prot
SynonymsST3O; SIAT4A; SIATFL; ST3GalA; ST3GalIA; Gal-NAc6S; ST3GalA.1; 1; ST3GAL1
Calculated MW39kDa
Observed MW39kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse liver
Cellular locationGolgi apparatus, Golgi stack membrane, Secreted, Single-pass type II membrane protein
    ABclonal:Western blot - ST3GAL1 Rabbit pAb (A11616)}

    Western blot - ST3GAL1 Rabbit pAb (A11616)

    Western blot analysis of lysates from mouse liver, using ST3GAL1 Rabbit pAb (A11616) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 40s.

    * For research use only. Not for therapeutic or diagnostic purposes.