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STAP2 Rabbit pAb (A16131)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - STAP2 Rabbit pAb (A16131)

Western blot analysis of lysates from Mouse heart, using STAP2 Rabbit pAb (A16131) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - STAP2 Rabbit pAb (A16131)

Western blot analysis of lysates from HT-29 cells, using STAP2 Rabbit pAb (A16131) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 240s.

Overview

Product nameSTAP2 Rabbit pAb
Catalog No.A16131
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the substrate of breast tumor kinase, an Src-type non-receptor tyrosine kinase. The encoded protein possesses domains and several tyrosine phosphorylation sites characteristic of adaptor proteins that mediate the interactions linking proteins involved in signal transduction pathways. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 130-220 of human STAP2 (NP_001013863).
SequenceGHLYMMSEVLAKEEARRALETPSCFLKVSRLEAQLLLERYPECGNLLLRPSGDGADGVSVTTRQMHNGTHVVRHYKVKREGPKYVIDVEQP
Gene ID
Swiss Prot
SynonymsBKS; STAP2
Calculated MW45kDa
Observed MW44kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse heart, HT-29
Cellular locationCytoplasm
    ABclonal:Western blot - STAP2 Rabbit pAb (A16131)}

    Western blot - STAP2 Rabbit pAb (A16131)

    Western blot analysis of lysates from Mouse heart, using STAP2 Rabbit pAb (A16131) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Western blot - STAP2 Rabbit pAb (A16131)}

    Western blot - STAP2 Rabbit pAb (A16131)

    Western blot analysis of lysates from HT-29 cells, using STAP2 Rabbit pAb (A16131) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 240s.

    * For research use only. Not for therapeutic or diagnostic purposes.