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STAT6 Rabbit pAb (A0755)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using STAT6 Rabbit pAb (A0755) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - STAT6 Rabbit pAb (A0755)

Immunofluorescence analysis of HeLa cells using STAT6 Rabbit pAb (A0755) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using STAT6 Rabbit pAb (A0755) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using STAT6 Rabbit pAb (A0755) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using STAT6 Rabbit pAb (A0755) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using STAT6 Rabbit pAb (A0755) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using STAT6 Rabbit pAb (A0755) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	STAT6 Rabbit pAb
Catalog No.	A0755
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 501-600 of human STAT6 (NP_003144.3).
Sequence	QFNKEILLGRGFTFWQWFDGVLDLTKRCLRSYWSDRLIIGFISKQYVTSLLLNEPDGTFLLRFSDSEIGGITIAHVIRGQDGSPQIENIQPFSAKDLSIRSLGDRIRDLAQLKNLYPKKPKDEAFRSHYKPEQMGKDGRGYVPATIKMTVERDQPLPTPELQMPTMVPSYDLGMAPDSSMSMQLGPDMVPQVYPPHSHSIPPYQGLSPEESVNVLSAFQEPHLQMPPSLGQMSLPFDQPHPQGLLPCQPQEHAVSSPDPLLCSDVTMVEDSCLSQPVTAFPQGTWIGEDIFPPLLPPTEQDLTKLLLEGQGESGGGSLGAQPLLQPSHYGQSGISMSHMDLRANPSW
Gene ID
Swiss Prot
Synonyms	STAT6B; STAT6C; D12S1644; IL-4-STAT; STAT6
Calculated MW	94kDa
Observed MW	110kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HeLa, Jurkat, PC-12, NIH/3T3, Mouse lung, Mouse spleen, Rat lung
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Mus musculus, Homo sapiens, Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

STAT6 Rabbit pAb images

Western blot - STAT6 Rabbit pAb (A0755)

Immunofluorescence - STAT6 Rabbit pAb (A0755)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for STAT6 Rabbit pAb (A0755)

Product:

STAT6 Rabbit pAb

Journal:

The Journal of Experimental Medicine

Application:

IF:

12.6

Species:

Mus musculus

PMID:

29970474

Title:

Inhibition of CRTH2-mediated Th2 activation attenuates pulmonary hypertension in mice.

References for STAT6 Rabbit pAb (A0755)

Product:

STAT6 Rabbit pAb

Journal:

Journal of Diabetes Research

Application:

IF:

4.3

Species:

Mus musculus

PMID:

31886281

Title:

Static Magnetic Field Accelerates Diabetic Wound Healing by Facilitating Resolution of Inflammation

References for STAT6 Rabbit pAb (A0755)

Product:

STAT6 Rabbit pAb

Journal:

PLoS ONE

Application:

IF:

2.9

Species:

Homo sapiens

PMID:

27253400

Title:

Finasteride Enhances the Generation of Human Myeloid-Derived Suppressor Cells by Up-Regulating the COX2/PGE2 Pathway.

References for STAT6 Rabbit pAb (A0755)

Product:

STAT6 Rabbit pAb

Journal:

Advanced healthcare materials

Application:

IF:

Species:

Mus musculus

PMID:

36603196

Title:

Antifibrotic Effects of Tetrahedral Framework Nucleic Acids by Inhibiting Macrophage Polarization and Macrophage‐myofibroblast Transition in Bladder Remodeling

References for STAT6 Rabbit pAb (A0755)

Product:

STAT6 Rabbit pAb

Journal:

Immunological investigations

Application:

IF:

2.9

Species:

Mus musculus

PMID:

36731128

Title:

All-Trans Retinoic Acid Promotes M2 Macrophage Polarization in Vitro by Activating the p38MAPK/STAT6 Signaling Pathway

References for STAT6 Rabbit pAb (A0755)

Product:

STAT6 Rabbit pAb

Journal:

BMC immunology

Application:

IF:

3.6

Species:

Mus musculus

PMID:

38172698

Title:

PRMT2 silencing regulates macrophage polarization through activation of STAT1 or inhibition of STAT6 - PMC

References for STAT6 Rabbit pAb (A0755)

Product:

STAT6 Rabbit pAb

Journal:

BMC immunology

Application:

IF:

3.6

Species:

Mus musculus

PMID:

38172698

Title:

PRMT2 silencing regulates macrophage polarization through activation of STAT1 or inhibition of STAT6 - PMC

References for STAT6 Rabbit pAb (A0755)

Product:

STAT6 Rabbit pAb

Journal:

Journal of ethnopharmacology

Application:

IF:

4.8

Species:

Rattus norvegicus

PMID:

36209953

Title:

Wutou decoction attenuates the synovial inflammation of collagen-induced arthritis rats via regulating macrophage M1/M2 type polarization

References for STAT6 Rabbit pAb (A0755)

Product:

STAT6 Rabbit pAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

IF:

15.1

Species:

Mus musculus

PMID:

37400369

Title:

LiverInspired Polyetherketoneketone Scaffolds Simulate Regenerative Signals and Mobilize AntiInflammatory Reserves to Reprogram Macrophage Metabolism for

References for STAT6 Rabbit pAb (A0755)

Product:

STAT6 Rabbit pAb

Journal:

Neuroscience

Application:

IF:

2.9

Species:

Rattus norvegicus

PMID:

38782114

Title:

Glycoprotein non-metastatic melanoma protein B (GPNMB) protects against neuroinflammation and neuronal loss in pilocarpine-induced epilepsy via the regulation of

References for STAT6 Rabbit pAb (A0755)

Product:

STAT6 Rabbit pAb

Journal:

Science advances

Application:

IF:

11.7

Species:

Mus musculus

PMID:

38924414