제품 > 항체 > 다중클론항체(pAb)

STIP1 Rabbit pAb (A14106)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat, Monkey

Western blot analysis of various lysates using STIP1 Rabbit pAb (A14106) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - STIP1 Rabbit pAb (A14106)

Immunohistochemistry analysis of paraffin-embedded rat testis using STIP1 Rabbit pAb (A14106) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse testis using STIP1 Rabbit pAb (A14106) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - STIP1 Rabbit pAb (A14106)

Immunofluorescence analysis of HeLa cells using STIP1 Rabbit pAb (A14106). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - STIP1 Rabbit pAb (A14106)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 1 μg STIP1 antibody ( A14106). Western blot was performed from the immunoprecipitate using STIP1 antibody ( A14106) at a dilution of 1:1000.

Overview

Product name	STIP1 Rabbit pAb
Catalog No.	A14106
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human STIP1 (NP_006810.1).
Sequence	MEQVNELKEKGNKALSVGNIDDALQCYSEAIKLDPHNHVLYSNRSAAYAKKGDYQKAYEDGCKTVDLKPDWGKGYSRKAAALEFLNRFEEAKRTYEEGLKHEANNPQLKEGLQNMEARLAERKFMNPFNMPNLYQKLESDPRTRTLLSDPTYRELIEQLRNKPSDLGTKLQDPRIMTTLSVLLGVDLGSMDEEEEIATPPPPPPPKKETKPEPMEEDLPENKKQALKEKELGNDAYKKKDFDTALKHYDKAKELDPTNMTYITNQAAVYFEKGDYNKCRELCEKAIEVGRENREDYRQIA
Gene ID
Swiss Prot
Synonyms	HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521; STIP1
Calculated MW	63kDa
Observed MW	69kDa

Applications

Reactivity	Human, Mouse, Rat, Monkey
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	HeLa, COS-7, COS-1, OVCAR3, Mouse brain
Cellular location	Cytoplasm, Nucleus

Documents

Certificate of Compliance

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Research Area

연구후성유전학 & 핵 신호전달RNA 결합

STIP1 Rabbit pAb images

Western blot - STIP1 Rabbit pAb (A14106)

Immunohistochemistry - STIP1 Rabbit pAb (A14106)

Immunofluorescence - STIP1 Rabbit pAb (A14106)

Immunofluorescence analysis of HeLa cells using STIP1 Rabbit pAb (A14106). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunoprecipitation - STIP1 Rabbit pAb (A14106)

* For research use only. Not for therapeutic or diagnostic purposes.