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STK24 Rabbit pAb (A10576)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - STK24 Rabbit pAb (A10576)

Western blot analysis of various lysates using STK24 Rabbit pAb (A10576) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - STK24 Rabbit pAb (A10576)

Immunohistochemistry analysis of paraffin-embedded Rat lung using STK24 Rabbit pAb (A10576) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - STK24 Rabbit pAb (A10576)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using STK24 Rabbit pAb (A10576) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - STK24 Rabbit pAb (A10576)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using STK24 Rabbit pAb (A10576) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - STK24 Rabbit pAb (A10576)

Immunofluorescence analysis of HeLa cells using STK24 Rabbit pAb (A10576) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - STK24 Rabbit pAb (A10576)

Immunofluorescence analysis of NIH/3T3 cells using STK24 Rabbit pAb (A10576) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - STK24 Rabbit pAb (A10576)

Immunofluorescence analysis of PC-12 cells using STK24 Rabbit pAb (A10576) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameSTK24 Rabbit pAb
Catalog No.A10576
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a serine/threonine protein kinase that functions upstream of mitogen-activated protein kinase (MAPK) signaling. The encoded protein is cleaved into two chains by caspases; the N-terminal fragment (MST3/N) translocates to the nucleus and promotes programmed cells death. There is a pseudogene for this gene on chromosome X. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 312-431 of human STK24 (NP_001027467.2).
SequenceTDGQASGGSDSGDWIFTIREKDPKNLENGALQPSDLDRNKMKDIPKRPFSQCLSTIISPLFAELKEKSQACGGNLGSIEELRGAIYLAEEACPGISDTMVAQLVQRLQRYSLSGGGTSSH
Gene ID
Swiss Prot
SynonymsMST3; STK3; MST3B; STE20; HEL-S-95; STK24
Calculated MW49kDa
Observed MW49kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, A375, U-251MG, HT-29, NIH/3T3, Mouse liver, Mouse kidney, Mouse lung, Rat liver
Cellular locationCytoplasm, Membrane, Nucleus.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - STK24 Rabbit pAb (A10576)}

    Western blot - STK24 Rabbit pAb (A10576)

    Western blot analysis of various lysates using STK24 Rabbit pAb (A10576) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - STK24 Rabbit pAb (A10576)}

    Immunohistochemistry - STK24 Rabbit pAb (A10576)

    Immunohistochemistry analysis of paraffin-embedded Rat lung using STK24 Rabbit pAb (A10576) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - STK24 Rabbit pAb (A10576)}

    Immunohistochemistry - STK24 Rabbit pAb (A10576)

    Immunohistochemistry analysis of paraffin-embedded Human tonsil using STK24 Rabbit pAb (A10576) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - STK24 Rabbit pAb (A10576)}

    Immunohistochemistry - STK24 Rabbit pAb (A10576)

    Immunohistochemistry analysis of paraffin-embedded Mouse spleen using STK24 Rabbit pAb (A10576) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunofluorescence - STK24 Rabbit pAb (A10576)}

    Immunofluorescence - STK24 Rabbit pAb (A10576)

    Immunofluorescence analysis of HeLa cells using STK24 Rabbit pAb (A10576) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - STK24 Rabbit pAb (A10576)}

    Immunofluorescence - STK24 Rabbit pAb (A10576)

    Immunofluorescence analysis of NIH/3T3 cells using STK24 Rabbit pAb (A10576) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - STK24 Rabbit pAb (A10576)}

    Immunofluorescence - STK24 Rabbit pAb (A10576)

    Immunofluorescence analysis of PC-12 cells using STK24 Rabbit pAb (A10576) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)