제품 > 항체 > 다중클론항체(pAb)

STK38L Rabbit pAb (A15406)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - STK38L Rabbit pAb (A15406)

Western blot analysis of various lysates using STK38L Rabbit pAb (A15406) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - STK38L Rabbit pAb (A15406)

Immunofluorescence analysis of L929 cells using STK38L Rabbit pAb (A15406) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameSTK38L Rabbit pAb
Catalog No.A15406
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables ATP binding activity; magnesium ion binding activity; and protein serine/threonine kinase activity. Involved in intracellular signal transduction. Acts upstream of or within protein phosphorylation. Located in cytosol.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 165-464 of human STK38L (NP_055815.1).
SequenceLIMEFLPGGDMMTLLMKKDTLTEEETQFYISETVLAIDAIHQLGFIHRDIKPDNLLLDAKGHVKLSDFGLCTGLKKAHRTEFYRNLTHNPPSDFSFQNMNSKRKAETWKKNRRQLAYSTVGTPDYIAPEVFMQTGYNKLCDWWSLGVIMYEMLIGYPPFCSETPQETYRKVMNWKETLVFPPEVPISEKAKDLILRFCIDSENRIGNSGVEEIKGHPFFEGVDWEHIRERPAAIPIEIKSIDDTSNFDDFPESDILQPVPNTTEPDYKSKDWVFLNYTYKRFEGLTQRGSIPTYMKAGKL
Gene ID
Swiss Prot
SynonymsNDR2; STK38L
Calculated MW54kDa
Observed MW54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, Mouse small intestine, Mouse brain, Rat stomach, Rat brain
Cellular locationCytoplasm, Membrane, cytoskeleton

Documents

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    ABclonal:Western blot - STK38L Rabbit pAb (A15406)}

    Western blot - STK38L Rabbit pAb (A15406)

    Western blot analysis of various lysates using STK38L Rabbit pAb (A15406) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunofluorescence - STK38L Rabbit pAb (A15406)}

    Immunofluorescence - STK38L Rabbit pAb (A15406)

    Immunofluorescence analysis of L929 cells using STK38L Rabbit pAb (A15406) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.