제품 > 항체 > 다중클론항체(pAb)

STX6 Rabbit pAb (A16283)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - STX6 Rabbit pAb (A16283)

Western blot analysis of various lysates, using STX6 Rabbit pAb (A16283) at 1:800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameSTX6 Rabbit pAb
Catalog No.A16283
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables syntaxin binding activity. Involved in several processes, including Golgi ribbon formation; retrograde transport, endosome to Golgi; and vesicle fusion. Acts upstream of or within endocytic recycling. Located in several cellular components, including early endosome; perinuclear region of cytoplasm; and trans-Golgi network. Part of SNARE complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-234 of human STX6 (NP_005810.1).
SequenceMSMEDPFFVVKGEVQKAVNTAQGLFQRWTELLQDPSTATREEIDWTTNELRNNLRSIEWDLEDLDETISIVEANPRKFNLDATELSIRKAFITSTRQVVRDMKDQMSTSSVQALAERKNRQALLGDSGSQNWSTGTTDKYGRLDRELQRANSHFIEEQQAQQQLIVEQQDEQLELVSGSIGVLKNMSQRIGGELEEQAVMLEDFSHELESTQSRLDNVMKKLAKVSHMTSDRRQ
Gene ID
Swiss Prot
SynonymsSTX6
Calculated MW29kDa
Observed MW35kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesbewo, SK-BR-3, Mouse brain, Mouse thymus
Cellular locationGolgi apparatus membrane, Single-pass type IV membrane protein.

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Lot number

    ABclonal:Western blot - STX6 Rabbit pAb (A16283)}

    Western blot - STX6 Rabbit pAb (A16283)

    Western blot analysis of various lysates, using STX6 Rabbit pAb (A16283) at 1:800 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)

    HRP-conjugated Goat anti-Rabbit IgG (H+L)
    AS014

    WBDBIHC-PELISA

    HRP-conjugated Goat anti-Rabbit IgG (H+L)

    FITC-conjugated Goat anti-Rabbit IgG (H+L)
    AS011

    IF/ICCFC

    FITC-conjugated Goat anti-Rabbit IgG (H+L)

    Cy3-conjugated Goat anti-Rabbit IgG (H+L)
    AS007

    IF/ICCFC

    Cy3-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
    AS053

    IF/ICCFC

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain
    AS061

    WBIP

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain

    HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
    AS063

    WB

    HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

    ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
    AS039

    IF/ICCFC

    ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

    Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
    AS040

    IHC-PIF/ICCFC

    Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
    AS060

    IF/ICCFC

    ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

    Goat Anti-Rabbit IgG(H+L)
    AS070

    WBELISACUT&Tag

    Goat Anti-Rabbit IgG(H+L)

    HRP-conjugated Donkey anti-Rabbit IgG (H+L)
    AS038

    WBIHC-P

    HRP-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
    AS035

    IF/ICCFC

    ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
    AS073

    IF/ICCFC

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
    AS058

    IF/ICCFC

    ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

    FITC-conjugated Donkey anti-Rabbit IgG (H+L)
    AS042

    IF/ICCFC

    FITC-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
    AS056

    IF/ICCFC

    ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

    AP-conjugated Goat anti-Rabbit IgG (H+L)
    AS009

    WB

    AP-conjugated Goat anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG (H+L)
    AS079

    WB

    HRP-conjugated Mouse anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
    AS126

    IPELISA

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

    PE Mouse anti-Rabbit IgG, light chain specific mAb
    AS129

    FC

    PE Mouse anti-Rabbit IgG, light chain specific mAb

    HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
    AS122

    WBELISA

    HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

    Rabbit Anti-Chicken IgY (H+L)
    AS090

    WB

    Rabbit Anti-Chicken IgY (H+L)

    Rabbit Anti-Chicken IgY Fab
    AS088

    WB

    Rabbit Anti-Chicken IgY Fab

    FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
    AS083

    IF/ICCFC

    FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

    Rabbit Anti-Chicken IgY (H+L)
    AS082

    WB

    Rabbit Anti-Chicken IgY (H+L)

    ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
    AS072

    IF/ICCFC

    ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)