제품 > 항체 > 다중클론항체(pAb)

SUFU Rabbit pAb (A13429)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Mouse testis using SUFU Rabbit pAb(A13429) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - SUFU Rabbit pAb (A13429)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using SUFU Rabbit pAb (A13429) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using SUFU Rabbit pAb (A13429) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - SUFU Rabbit pAb (A13429)

Immunofluorescence analysis of MCF-7 cells using SUFU Rabbit pAb (A13429). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	SUFU Rabbit pAb
Catalog No.	A13429
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 325-484 of human SUFU (NP_057253.2).
Sequence	PPINPQRQNGLAHDRAPSRKDSLESDSSTAIIPHELIRTRQLESVHLKFNQESGALIPLCLRGRLLHGRHFTYKSITGDMAITFVSTGVEGAFATEEHPYAAHGPWLQILLTEEFVEKMLEDLEDLTSPEEFKLPKEYSWPEKKLKVSILPDVVFDSPLH
Gene ID
Swiss Prot
Synonyms	SUFUH; JBTS32; SUFUXL; PRO1280; SUFU
Calculated MW	54kDa
Observed MW	54kDa/

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	C6, PC-12, Mouse testis
Cellular location	Cytoplasm, Nucleus

Documents

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Research Area

연구세포 생물학 & 발달 생물학헤지호그 신호전달 경로

SUFU Rabbit pAb images

Western blot - SUFU Rabbit pAb (A13429)

Immunohistochemistry - SUFU Rabbit pAb (A13429)

Immunofluorescence - SUFU Rabbit pAb (A13429)

Immunofluorescence analysis of MCF-7 cells using SUFU Rabbit pAb (A13429). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.