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SUMO1 Rabbit pAb (A2130)

Publications (8) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SUMO1 Rabbit pAb (A2130)

Western blot analysis of various lysates using SUMO1 Rabbit pAb (A2130) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - SUMO1 Rabbit pAb (A2130)

Immunofluorescence analysis of HeLa cells using SUMO1 Rabbit pAb (A2130) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SUMO1 Rabbit pAb (A2130)

Immunofluorescence analysis of A549 cells using SUMO1 Rabbit pAb (A2130) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SUMO1 Rabbit pAb (A2130)

Immunofluorescence analysis of MCF7 cells using SUMO1 Rabbit pAb (A2130) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameSUMO1 Rabbit pAb
Catalog No.A2130
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human SUMO1 (NP_003343.1).
SequenceMSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGHSTV
Gene ID
Swiss Prot
SynonymsDAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3; SUMO1
Calculated MW12kDa
Observed MW17kDa/80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Jurkat
Cellular locationCell membrane, Cytoplasm, Nucleus, Nucleus membrane, Nucleus speckle, PML body
Customer validation

WB(Homo sapiens, Rattus norvegicus)

IP(Homo sapiens, Rattus norvegicus)

IF(Drosophila melanogaster)

IP(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - SUMO1 Rabbit pAb (A2130)}

Western blot - SUMO1 Rabbit pAb (A2130)

Western blot analysis of various lysates using SUMO1 Rabbit pAb (A2130) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - SUMO1 Rabbit pAb (A2130)}

Immunofluorescence - SUMO1 Rabbit pAb (A2130)

Immunofluorescence analysis of HeLa cells using SUMO1 Rabbit pAb (A2130) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SUMO1 Rabbit pAb (A2130)}

Immunofluorescence - SUMO1 Rabbit pAb (A2130)

Immunofluorescence analysis of A549 cells using SUMO1 Rabbit pAb (A2130) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SUMO1 Rabbit pAb (A2130)}

Immunofluorescence - SUMO1 Rabbit pAb (A2130)

Immunofluorescence analysis of MCF7 cells using SUMO1 Rabbit pAb (A2130) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A2130? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)