제품 > 항체 > 다중클론항체(pAb)

SYN1 Rabbit pAb (A5247)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SYN1 Rabbit pAb (A5247)

Western blot analysis of SH-SY5Y, using SYN1 Rabbit pAb (A5247) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunofluorescence - SYN1 Rabbit pAb (A5247)

Immunofluorescence analysis of mouse brain using SYN1 Rabbit pAb (A5247) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SYN1 Rabbit pAb (A5247)

Immunofluorescence analysis of rat brain using SYN1 Rabbit pAb (A5247) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameSYN1 Rabbit pAb
Catalog No.A5247
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 400-500 of human SYN1 (NP_008881.2).
SequenceDEDKQLIVELVVNKMAQALPRQRQRDASPGRGSHGQTPSPGALPLGRQTSQQPAGPPAQQRPPPQGGPPQPGPGPQRQGPPLQQRPPPQGQQHLSGLGPPA
Gene ID
Swiss Prot
SynonymsSYNI; EPILX; MRX50; SYN1a; SYN1b; EPILX1
Calculated MW74kDa
Observed MW77kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesSH-SY5Y
Cellular locationCell junction, Golgi apparatus, synapse

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ABclonal:Western blot - SYN1 Rabbit pAb (A5247)}

Western blot - SYN1 Rabbit pAb (A5247)

Western blot analysis of SH-SY5Y, using SYN1 Rabbit pAb (A5247) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunofluorescence - SYN1 Rabbit pAb (A5247)}

Immunofluorescence - SYN1 Rabbit pAb (A5247)

Immunofluorescence analysis of mouse brain using SYN1 Rabbit pAb (A5247) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SYN1 Rabbit pAb (A5247)}

Immunofluorescence - SYN1 Rabbit pAb (A5247)

Immunofluorescence analysis of rat brain using SYN1 Rabbit pAb (A5247) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

ELISA 키트 (1)

Secondary Antibodies (26)