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SYNE3 Rabbit pAb (A13237)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - SYNE3 Rabbit pAb (A13237)

Western blot analysis of various lysates using SYNE3 Rabbit pAb (A13237) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - SYNE3 Rabbit pAb (A13237)

Immunofluorescence analysis of HeLa cells using SYNE3 Rabbit pAb (A13237) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SYNE3 Rabbit pAb (A13237)

Immunofluorescence analysis of NIH/3T3 cells using SYNE3 Rabbit pAb (A13237) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameSYNE3 Rabbit pAb
Catalog No.A13237
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables actin filament binding activity and cytoskeleton-nuclear membrane anchor activity. Involved in cytoskeleton organization; establishment of protein localization to membrane; and regulation of cell shape. Located in nuclear membrane. Part of meiotic nuclear membrane microtubule tethering complex. Biomarker of Huntington's disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 740-920 of human SYNE3 (NP_689805.3).
SequenceLRLLEESLLSLIRNWHLQRMEVDSGKKMVFTNNIPKSGFLINPMDPIPRHRRRANLLQEEEGSHEDFSQLLRNFGQWLQVENSKLVRIIAMRTSTAEDLRTRKSKLQELEARVPEGQHLFENLLRLGPARGTSDELEDLRYQWMLYKSKLKDSGHLLTQSSPGEPTGFQKTRRWRGLGSLF
Gene ID
Swiss Prot
SynonymsKASH3; NET53; Nesp3; C14orf49; C14orf139; LINC00341; NCRNA00341; SYNE3
Calculated MW112kDa
Observed MW112kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesJurkat, K-562, LO2, HepG2
Cellular locationNucleus envelope, Nucleus outer membrane, Rough endoplasmic reticulum, Single-pass type IV membrane protein

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ABclonal:Western blot - SYNE3 Rabbit pAb (A13237)}

Western blot - SYNE3 Rabbit pAb (A13237)

Western blot analysis of various lysates using SYNE3 Rabbit pAb (A13237) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - SYNE3 Rabbit pAb (A13237)}

Immunofluorescence - SYNE3 Rabbit pAb (A13237)

Immunofluorescence analysis of HeLa cells using SYNE3 Rabbit pAb (A13237) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SYNE3 Rabbit pAb (A13237)}

Immunofluorescence - SYNE3 Rabbit pAb (A13237)

Immunofluorescence analysis of NIH/3T3 cells using SYNE3 Rabbit pAb (A13237) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)