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Slit2 Rabbit mAb (A3467)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Slit2 Rabbit mAb (A3467)

Western blot analysis of various lysates using Slit2 Rabbit mAb (A3467) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - Slit2 Rabbit mAb (A3467)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using Slit2 Rabbit mAb (A3467) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product nameSlit2 Rabbit mAb
Catalog No.A3467
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2010
This gene encodes a member of the slit family of secreted glycoproteins, which are ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. Members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Proteolytic processing of this protein gives rise to an N-terminal fragment that contains the four leucine-rich repeats and five epidermal growth factor repeats and a C-terminal fragment that contains four epidermal growth factor repeats and the cysteine knot. Both full length and cleaved proteins are secreted extracellularly and can function in axon repulsion as well as other specific processes. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1400-1500 of human Slit2 (O94813).
SequenceGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRR
Gene ID
Swiss Prot
SynonymsSLIL3; Slit-2; Slit2
Calculated MW170kDa
Observed MW200kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, PC-3, Mouse lung, Mouse brain, Mouse kidney, Rat brain
Cellular locationSecreted.
Customer validation

IF(Mus musculus)

WB(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - Slit2 Rabbit mAb (A3467)}

    Western blot - Slit2 Rabbit mAb (A3467)

    Western blot analysis of various lysates using Slit2 Rabbit mAb (A3467) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3min.
    ABclonal:Immunohistochemistry - Slit2 Rabbit mAb (A3467)}

    Immunohistochemistry - Slit2 Rabbit mAb (A3467)

    Immunohistochemistry analysis of paraffin-embedded Rat ovary using Slit2 Rabbit mAb (A3467) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A3467? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    Secondary Antibodies (26)