제품 > 항체 > 다중클론항체(pAb)

SnRK2.3 Rabbit pAb (A21281)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Arabidopsis thaliana

ABclonal:Western blot - SnRK2.3 Rabbit pAb (A21281)

Western blot analysis of lysates from Inflorescence, using SnRK2.3 Rabbit pAb (A21281) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product nameSnRK2.3 Rabbit pAb
Catalog No.A21281
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
encodes a member of SNF1-related protein kinases (SnRK2) whose activity is activated by ionic (salt) and non-ionic (mannitol) osmotic stress. Enzyme involved in the ABA signaling during seed germination, dormancy and seedling growth.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-300 of arabidopsis thaliana SnRK2.3 (NP_201489.1).
SequenceDVWSCGVTLYVMLVGAYPFEDPEEPRDYRKTIQRILSVKYSIPDDIRISPECCHLISRIFVADPATRISIPEIKTHSWFLKNLPADLMNESNTGSQFQEPE
Gene ID
Swiss Prot
SynonymsSNRK2-3; SRK2I; SUCROSE NONFERMENTING 1 (SNF1)-RELATED PROTEIN KINASE 2-3; sucrose nonfermenting 1(SNF1)-related protein kinase 2.3; SnRK2.3
Calculated MW41kDa
Observed MW41kDa
ReactivityArabidopsis thaliana
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesInflorescence
Cellular locationcytoplasm, nucleus, plastid
    ABclonal:Western blot - SnRK2.3 Rabbit pAb (A21281)}

    Western blot - SnRK2.3 Rabbit pAb (A21281)

    Western blot analysis of lysates from Inflorescence, using SnRK2.3 Rabbit pAb (A21281) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.