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Sterol carrier protein 2 Rabbit mAb (A9430)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Sterol carrier protein 2 Rabbit mAb (A9430)

Western blot analysis of various lysates using (A9430) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - Sterol carrier protein 2 Rabbit mAb (A9430)

Immunohistochemistry analysis of Sterol carrier protein 2 in paraffin-embedded rat colon tissue using Sterol carrier protein 2 Rabbit mAb (A9430) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Sterol carrier protein 2 Rabbit mAb (A9430)

Immunohistochemistry analysis of Sterol carrier protein 2 in paraffin-embedded human liver tissue using Sterol carrier protein 2 Rabbit mAb (A9430) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Sterol carrier protein 2 Rabbit mAb (A9430)

Immunohistochemistry analysis of Sterol carrier protein 2 in paraffin-embedded human colon carcinoma tissue using Sterol carrier protein 2 Rabbit mAb (A9430) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Sterol carrier protein 2 Rabbit mAb (A9430)

Immunohistochemistry analysis of Sterol carrier protein 2 in paraffin-embedded mouse brain tissue using Sterol carrier protein 2 Rabbit mAb (A9430) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Sterol carrier protein 2 Rabbit mAb (A9430)

Immunohistochemistry analysis of Sterol carrier protein 2 in paraffin-embedded human spleen tissue using Sterol carrier protein 2 Rabbit mAb (A9430) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Sterol carrier protein 2 Rabbit mAb (A9430)

Immunofluorescence analysis of HepG2 cells using Sterol carrier protein 2 Rabbit mAb (A9430) at dilution of 1:50. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Sterol carrier protein 2 Rabbit mAb (A9430)

Immunofluorescence analysis of U2OS cells using Sterol carrier protein 2 Rabbit mAb (A9430) at dilution of 1:50. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Sterol carrier protein 2 Rabbit mAb (A9430)

Immunofluorescence analysis of NIH/3T3 cells using Sterol carrier protein 2 Rabbit mAb (A9430) at dilution of 1:50. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Sterol carrier protein 2 Rabbit mAb (A9430)

Immunofluorescence analysis of PC-12 cells using Sterol carrier protein 2 Rabbit mAb (A9430) at dilution of 1:50. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameSterol carrier protein 2 Rabbit mAb
Catalog No.A9430
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2735
This gene encodes two proteins: sterol carrier protein X (SCPx) and sterol carrier protein 2 (SCP2), as a result of transcription initiation from 2 independently regulated promoters. The transcript initiated from the proximal promoter encodes the longer SCPx protein, and the transcript initiated from the distal promoter encodes the shorter SCP2 protein, with the 2 proteins sharing a common C-terminus. Evidence suggests that the SCPx protein is a peroxisome-associated thiolase that is involved in the oxidation of branched chain fatty acids, while the SCP2 protein is thought to be an intracellular lipid transfer protein. This gene is highly expressed in organs involved in lipid metabolism, and may play a role in Zellweger syndrome, in which cells are deficient in peroxisomes and have impaired bile acid synthesis. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Sterol carrier protein 2 (P22307).
SequenceMSSSPWEPATLRRVFVVGVGMTKFVKPGAENSRDYPDLAEEAGKKALADAQIPYSAVDQACVGYVFGDSTCGQRAIYHSLGMTGIPIINVNNNCATGSTA
Gene ID
Swiss Prot
SynonymsNLTP; SCOX; SCPX; SCP-2; SCP-X; NSL-TP; SCP-CHI; Sterol carrier protein 2
Calculated MW59kDa
Observed MW59kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, Hep G2, U-87MG, Rat liver, Rat brain
Cellular locationCytoplasm, Mitochondrion, Mitochondrion, Peroxisome
Customer validation

IHC(Mus musculus )

IF(Mus musculus )

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Sterol carrier protein 2 Rabbit mAb (A9430)}

    Western blot - Sterol carrier protein 2 Rabbit mAb (A9430)

    Western blot analysis of various lysates using (A9430) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunohistochemistry - Sterol carrier protein 2 Rabbit mAb (A9430)}

    Immunohistochemistry - Sterol carrier protein 2 Rabbit mAb (A9430)

    Immunohistochemistry analysis of Sterol carrier protein 2 in paraffin-embedded rat colon tissue using Sterol carrier protein 2 Rabbit mAb (A9430) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Sterol carrier protein 2 Rabbit mAb (A9430)}

    Immunohistochemistry - Sterol carrier protein 2 Rabbit mAb (A9430)

    Immunohistochemistry analysis of Sterol carrier protein 2 in paraffin-embedded human liver tissue using Sterol carrier protein 2 Rabbit mAb (A9430) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Sterol carrier protein 2 Rabbit mAb (A9430)}

    Immunohistochemistry - Sterol carrier protein 2 Rabbit mAb (A9430)

    Immunohistochemistry analysis of Sterol carrier protein 2 in paraffin-embedded human colon carcinoma tissue using Sterol carrier protein 2 Rabbit mAb (A9430) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Sterol carrier protein 2 Rabbit mAb (A9430)}

    Immunohistochemistry - Sterol carrier protein 2 Rabbit mAb (A9430)

    Immunohistochemistry analysis of Sterol carrier protein 2 in paraffin-embedded mouse brain tissue using Sterol carrier protein 2 Rabbit mAb (A9430) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Sterol carrier protein 2 Rabbit mAb (A9430)}

    Immunohistochemistry - Sterol carrier protein 2 Rabbit mAb (A9430)

    Immunohistochemistry analysis of Sterol carrier protein 2 in paraffin-embedded human spleen tissue using Sterol carrier protein 2 Rabbit mAb (A9430) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - Sterol carrier protein 2 Rabbit mAb (A9430)}

    Immunofluorescence - Sterol carrier protein 2 Rabbit mAb (A9430)

    Immunofluorescence analysis of HepG2 cells using Sterol carrier protein 2 Rabbit mAb (A9430) at dilution of 1:50. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Sterol carrier protein 2 Rabbit mAb (A9430)}

    Immunofluorescence - Sterol carrier protein 2 Rabbit mAb (A9430)

    Immunofluorescence analysis of U2OS cells using Sterol carrier protein 2 Rabbit mAb (A9430) at dilution of 1:50. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Sterol carrier protein 2 Rabbit mAb (A9430)}

    Immunofluorescence - Sterol carrier protein 2 Rabbit mAb (A9430)

    Immunofluorescence analysis of NIH/3T3 cells using Sterol carrier protein 2 Rabbit mAb (A9430) at dilution of 1:50. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Sterol carrier protein 2 Rabbit mAb (A9430)}

    Immunofluorescence - Sterol carrier protein 2 Rabbit mAb (A9430)

    Immunofluorescence analysis of PC-12 cells using Sterol carrier protein 2 Rabbit mAb (A9430) at dilution of 1:50. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (25)