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Substance P Rabbit pAb (A20772)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Immunofluorescence - Substance P Rabbit pAb (A20772)

Immunofluorescence analysis of SH-SY5Y cells using Substance P Rabbit pAb (A20772) at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameSubstance P Rabbit pAb
Catalog No.A20772
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Substance P is an antimicrobial peptide with antibacterial and antifungal properties. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Substance P (NP_003173.1).
SequenceMKILVALAVFFLVSTQLFAEEIGANDDLNYWSDWYDSDQIKEELPEPFEHLLQRIARRPKPQQFFGLMGKRDADSSIEKQVALLKALYGHGQISHKRHKT
Gene ID
Swiss Prot
SynonymsNK2; NPK; NKNA; TAC2; Hs.2563; P
Calculated MW15kDa
Observed MW17kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:100 - 1:500
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T
Cellular locationaxon, extracellular region, extracellular space, synapse.
Customer validation

WB(Capra hircus)

Documents

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    ABclonal:Immunofluorescence - Substance P Rabbit pAb (A20772)}

    Immunofluorescence - Substance P Rabbit pAb (A20772)

    Immunofluorescence analysis of SH-SY5Y cells using Substance P Rabbit pAb (A20772) at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A20772? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    Secondary Antibodies (26)