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Syndecan 3 Rabbit mAb (A20929)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Syndecan 3 Rabbit mAb (A20929)

Western blot analysis of various lysates using Syndecan 3 Rabbit mAb (A20929) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameSyndecan 3 Rabbit mAb
Catalog No.A20929
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2907
The protein encoded by this gene belongs to the syndecan proteoglycan family. It may play a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. Allelic variants of this gene have been associated with obesity.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Syndecan 3 (O75056).
SequenceMKPGPPHRAGAAHGAGAGAGAAAGPGARGLLLPPLLLLLLAGRAAGAQRWRSENFERPVDLEGSGDDDSFPDDELDDLYSGSGSGYFEQESGIETAMRFS
Gene ID
Swiss Prot
SynonymsSDCN; SYND3; Syndecan 3
Calculated MW45kDa
Observed MW70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-251MG, MCF7, A-549, Mouse lung, Mouse spleen, Rat lung
Cellular locationcell surface, Golgi lumen, lysosomal lumen, microspike, plasma membrane.
Customer validation

WB(Rattus norvegicus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - Syndecan 3 Rabbit mAb (A20929)}

    Western blot - Syndecan 3 Rabbit mAb (A20929)

    Western blot analysis of various lysates using Syndecan 3 Rabbit mAb (A20929) at 1:500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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