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TCAP Rabbit pAb (A13566)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TCAP Rabbit pAb (A13566)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with TCAP using TCAP Rabbit pAb(A13566) at 1:2000 dilution. 
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 20 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 1s.

Overview

Product nameTCAP Rabbit pAb
Catalog No.A13566
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Sarcomere assembly is regulated by the muscle protein titin. Titin is a giant elastic protein with kinase activity that extends half the length of a sarcomere. It serves as a scaffold to which myofibrils and other muscle related proteins are attached. This gene encodes a protein found in striated and cardiac muscle that binds to the titin Z1-Z2 domains and is a substrate of titin kinase, interactions thought to be critical to sarcomere assembly. Mutations in this gene are associated with limb-girdle muscular dystrophy type 2G.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-167 of human TCAP (NP_003664.1).
SequenceMATSELSCEVSEENCERREAFWAEWKDLTLSTRPEEGCSLHEEDTQRHETYHQQGQCQVLVQRSPWLMMRMGILGRGLQEYQLPYQRVLPLPIFTPAKMGATKEEREDTPIQLQELLALETALGGQCVDRQEVAEITKQLPPVVPVSKPGALRRSLSRSMSQEAQRG
Gene ID
Swiss Prot
SynonymsTELE; CMD1N; CMH25; T-cap; LGMD2G; LGMDR7; telethonin; TCAP
Calculated MW19kDa
Observed MW19kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T transfected with TCAP
Cellular locationCytoplasm, myofibril, sarcomere

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ABclonal:Western blot - TCAP Rabbit pAb (A13566)}

Western blot - TCAP Rabbit pAb (A13566)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with TCAP using TCAP Rabbit pAb(A13566) at 1:2000 dilution. 
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 20 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

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