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TCEB3 Rabbit pAb (A6994)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TCEB3 Rabbit pAb (A6994)

Western blot analysis of extracts of various cell lines, using TCEB3 antibody (A6994) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Immunofluorescence - TCEB3 Rabbit pAb (A6994)

Immunofluorescence analysis of MCF7 cells using TCEB3 antibody (A6994).

Overview

Product nameTCEB3 Rabbit pAb
Catalog No.A6994
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the protein elongin A, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 679-798 of human TCEB3 (NP_003189.2).
SequenceANKPKGRQAKMAFVNSVAKPPRDVRRRQEKFGTGGAAVPEKIKIKPAPYPMGSSHASASSISFNPSPEEPAYDGPSTSSAHLAPVVSSTVSYDPRKPTVKKIAPMMAKTIKAFKNRFSRR
Gene ID
Swiss Prot
SynonymsSIII; TCEB3; TCEB3A; SIII p110
Calculated MW90kDa
Observed MW105kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesBT-474, HeLa, Raji, Mouse testis
Cellular locationNucleus

Documents

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    ABclonal:Western blot - TCEB3 Rabbit pAb (A6994)}

    Western blot - TCEB3 Rabbit pAb (A6994)

    Western blot analysis of extracts of various cell lines, using TCEB3 antibody (A6994) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - TCEB3 Rabbit pAb (A6994)}

    Immunofluorescence - TCEB3 Rabbit pAb (A6994)

    Immunofluorescence analysis of MCF7 cells using TCEB3 antibody (A6994).

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)