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TCIRG1 Rabbit mAb (A24945)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TCIRG1 Rabbit mAb (A24945)

Western blot analysis of lysates from Mouse spleen, using TCIRG1 Rabbit mAb (A24945) at 1:4000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Immunohistochemistry - TCIRG1 Rabbit mAb (A24945)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using TCIRG1 Rabbit mAb (A24945) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TCIRG1 Rabbit mAb (A24945)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using TCIRG1 Rabbit mAb (A24945) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TCIRG1 Rabbit mAb (A24945)

Immunohistochemistry analysis of paraffin-embedded Human skin tissue using TCIRG1 Rabbit mAb (A24945) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - TCIRG1 Rabbit mAb (A24945)

Confocal imaging of paraffin-embedded mouse spleen using TCIRG1 Rabbit mAb (A24945, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.

ABclonal:Immunofluorescence - TCIRG1 Rabbit mAb (A24945)

Confocal imaging of paraffin-embedded rat spleen using TCIRG1 Rabbit mAb (A24945, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.

ABclonal:Immunofluorescence - TCIRG1 Rabbit mAb (A24945)

Confocal imaging of paraffin-embedded THP-1 cells using TCIRG1 Rabbit mAb (A24945, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product nameTCIRG1 Rabbit mAb
Catalog No.A24945
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC64630
This gene encodes a subunit of a large protein complex known as a vacuolar H+-ATPase (V-ATPase). The protein complex acts as a pump to move protons across the membrane. This movement of protons helps regulate the pH of cells and their surrounding environment. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. Alternative splicing results in multiple transcript variants. Mutations in this gene are associated with infantile malignant osteopetrosis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to aminoacids 1-356 of human TCIRG1(NP_006010.2).
SequenceMGSMFRSEEVALVQLFLPTAAAYTCVSRLGELGLVEFRDLNASVSAFQRRFVVDVRRCEELEKTFTFLQEEVRRAGLVLPPPKGRLPAPPPRDLLRIQEETERLAQELRDVRGNQQALRAQLHQLQLHAAVLRQGHEPQLAAAHTDGASERTPLLQAPGGPHQDLRVNFVAGAVEPHKAPALERLLWRACRGFLIASFRELEQPLEHPVTGEPATWMTFLISYWGEQIGQKIRKITDCFHCHVFPFLQQEEARLGALQQLQQQSQELQEVLGETERFLSQVLGRVLQLLPPGQVQVHKMKAVYLALNQCSVSTTHKCLIAEAWCSVRDLPALQEALRDSSMEEGVSAVAHRIPCRD
Gene ID
Swiss Prot
Synonymsa3; Stv1; Vph1; Atp6i; OC116; OPTB1; TIRC7; ATP6N1C; ATP6V0A3; OC-116kDa; TCIRG1
Calculated MW93kDa
Observed MW116kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:8000
  • IHC-P 1:300 - 1:3000
  • IF/ICC 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse spleen
Cellular locationMembrane, Multi-pass membrane protein.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - TCIRG1 Rabbit mAb (A24945)}

    Western blot - TCIRG1 Rabbit mAb (A24945)

    Western blot analysis of lysates from Mouse spleen, using TCIRG1 Rabbit mAb (A24945) at 1:4000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 45s.
    ABclonal:Immunohistochemistry - TCIRG1 Rabbit mAb (A24945)}

    Immunohistochemistry - TCIRG1 Rabbit mAb (A24945)

    Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using TCIRG1 Rabbit mAb (A24945) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - TCIRG1 Rabbit mAb (A24945)}

    Immunohistochemistry - TCIRG1 Rabbit mAb (A24945)

    Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using TCIRG1 Rabbit mAb (A24945) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - TCIRG1 Rabbit mAb (A24945)}

    Immunohistochemistry - TCIRG1 Rabbit mAb (A24945)

    Immunohistochemistry analysis of paraffin-embedded Human skin tissue using TCIRG1 Rabbit mAb (A24945) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - TCIRG1 Rabbit mAb (A24945)}

    Immunofluorescence - TCIRG1 Rabbit mAb (A24945)

    Confocal imaging of paraffin-embedded mouse spleen using TCIRG1 Rabbit mAb (A24945, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.
    ABclonal:Immunofluorescence - TCIRG1 Rabbit mAb (A24945)}

    Immunofluorescence - TCIRG1 Rabbit mAb (A24945)

    Confocal imaging of paraffin-embedded rat spleen using TCIRG1 Rabbit mAb (A24945, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.
    ABclonal:Immunofluorescence - TCIRG1 Rabbit mAb (A24945)}

    Immunofluorescence - TCIRG1 Rabbit mAb (A24945)

    Confocal imaging of paraffin-embedded THP-1 cells using TCIRG1 Rabbit mAb (A24945, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

    * For research use only. Not for therapeutic or diagnostic purposes.

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