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TCIRG1 Rabbit pAb (A15382)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TCIRG1 Rabbit pAb (A15382)

Western blot analysis of lysates from Mouse spleen, using TCIRG1 Rabbit pAb (A15382) at 1:600 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - TCIRG1 Rabbit pAb (A15382)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using TCIRG1 Rabbit pAb (A15382) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - TCIRG1 Rabbit pAb (A15382)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using TCIRG1 Rabbit pAb (A15382) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - TCIRG1 Rabbit pAb (A15382)

Immunohistochemistry analysis of paraffin-embedded Human liver using TCIRG1 Rabbit pAb (A15382) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - TCIRG1 Rabbit pAb (A15382)

Immunofluorescence analysis of L929 cells using TCIRG1 Rabbit pAb (A15382) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - TCIRG1 Rabbit pAb (A15382)

Immunofluorescence analysis of U-2 OS cells using TCIRG1 Rabbit pAb (A15382) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameTCIRG1 Rabbit pAb
Catalog No.A15382
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a subunit of a large protein complex known as a vacuolar H+-ATPase (V-ATPase). The protein complex acts as a pump to move protons across the membrane. This movement of protons helps regulate the pH of cells and their surrounding environment. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. Alternative splicing results in multiple transcript variants. Mutations in this gene are associated with infantile malignant osteopetrosis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human TCIRG1 (NP_006010.2).
SequenceMGSMFRSEEVALVQLFLPTAAAYTCVSRLGELGLVEFRDLNASVSAFQRRFVVDVRRCEELEKTFTFLQEEVRRAGLVLPPPKGRLPAPPPRDLLRIQEETERLAQELRDVRGNQQALRAQLHQLQLHAA
Gene ID
Swiss Prot
Synonymsa3; Stv1; Vph1; Atp6i; OC116; OPTB1; TIRC7; ATP6N1C; ATP6V0A3; OC-116kDa; TCIRG1
Calculated MW93kDa
Observed MW110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse spleen
Cellular locationMembrane, Multi-pass membrane protein.
Customer validation

WB(Homo sapiens)

Other(Mus musculus,Oryctolagus cuniculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - TCIRG1 Rabbit pAb (A15382)}

    Western blot - TCIRG1 Rabbit pAb (A15382)

    Western blot analysis of lysates from Mouse spleen, using TCIRG1 Rabbit pAb (A15382) at 1:600 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 20s.
    ABclonal:Immunohistochemistry - TCIRG1 Rabbit pAb (A15382)}

    Immunohistochemistry - TCIRG1 Rabbit pAb (A15382)

    Immunohistochemistry analysis of paraffin-embedded Mouse spleen using TCIRG1 Rabbit pAb (A15382) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - TCIRG1 Rabbit pAb (A15382)}

    Immunohistochemistry - TCIRG1 Rabbit pAb (A15382)

    Immunohistochemistry analysis of paraffin-embedded Rat ovary using TCIRG1 Rabbit pAb (A15382) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - TCIRG1 Rabbit pAb (A15382)}

    Immunohistochemistry - TCIRG1 Rabbit pAb (A15382)

    Immunohistochemistry analysis of paraffin-embedded Human liver using TCIRG1 Rabbit pAb (A15382) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunofluorescence - TCIRG1 Rabbit pAb (A15382)}

    Immunofluorescence - TCIRG1 Rabbit pAb (A15382)

    Immunofluorescence analysis of L929 cells using TCIRG1 Rabbit pAb (A15382) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - TCIRG1 Rabbit pAb (A15382)}

    Immunofluorescence - TCIRG1 Rabbit pAb (A15382)

    Immunofluorescence analysis of U-2 OS cells using TCIRG1 Rabbit pAb (A15382) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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