제품 > 항체 > 다중클론항체(pAb)

TCTE1 Rabbit pAb (A15216)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TCTE1 Rabbit pAb (A15216)

Western blot analysis of various lysates using TCTE1 Rabbit pAb (A15216) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

Overview

Product nameTCTE1 Rabbit pAb
Catalog No.A15216
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to be involved in flagellated sperm motility. Predicted to be located in sperm flagellum.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TCTE1 (NP_872345.2).
SequenceMQDTVTTSALLDPSHSSVSTQDNSSTGGHTSSTSPQLSKPSITPVPAKSRNPHPRANIRRMRRIIAEDPEWSLAIVPLLTELCIQHIIRNFQKNPILKQMLPEHQQKVLNHLSPDLPLAVTANLIDSENYWLRCCMHRWPVCHVAHHGGSWKRMFFERHLENLLKHFIPGTTDPAVILDLLPLCRNYVRRVHVDQFLPPVQLPAQLRPGDQSDSGSEGEMEEPTVDHYQLGDLVAGLSHLEELDLVYDVKDCGMNFEWNLFLFTYRDCLSLAAAIKACHTLKIFKLTRSKVDDDKARIII
Gene ID
Swiss Prot
SynonymsDRC5; D6S46; FAP155; TCTE1
Calculated MW56kDa
Observed MW66kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSH-SY5Y, Mouse brain, Mouse testis, Mouse liver, Rat brain, Rat testis, Rat liver
Cellular locationcytoplasm, cytoskeleton, sperm flagellum

Documents

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    ABclonal:Western blot - TCTE1 Rabbit pAb (A15216)}

    Western blot - TCTE1 Rabbit pAb (A15216)

    Western blot analysis of various lysates using TCTE1 Rabbit pAb (A15216) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 15s.

    * For research use only. Not for therapeutic or diagnostic purposes.